High throughput sequencing (HTS) provides full repertoire analysis of the B and T cell receptors in humans and mice, both in blood and synovial tissue

Identification and monitoring of expanded clones has proved difficult owing to the extremely high diversity of T and B cell receptor (TCR/ BCR) repertoires. Peripheral blood mononuclear cells from two healthy donors (HD) and six Bl6 mice were analysed on a Genome Sequencer-FLX (Roche) providing >...

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Bibliographic Details
Published inAnnals of the rheumatic diseases Vol. 69; no. Suppl 2; p. A33
Main Authors Klarenbeek, P L, Tak, P P, Doorenspleet, M E, van Schaik, B D C, Wensveen, F M, Gottschal, L, Jakobs, M E, Derks, I A M, Eldering, E, van Kampen, A H C, Baas, F, de Vries, N
Format Journal Article
LanguageEnglish
Published London BMJ Publishing Group Ltd and European League Against Rheumatism 01.03.2010
BMJ Publishing Group LTD
Subjects
Cloning
Lymphocytes
T cell receptors
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Summary:Identification and monitoring of expanded clones has proved difficult owing to the extremely high diversity of T and B cell receptor (TCR/ BCR) repertoires. Peripheral blood mononuclear cells from two healthy donors (HD) and six Bl6 mice were analysed on a Genome Sequencer-FLX (Roche) providing >100 000 receptor sequences per run.
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ArticleID:A81
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ISSN:0003-4967
1468-2060
DOI:10.1136/ard.2010.129619o