OP-1 Conjunctival genetic ‘fingerprinting’ in ocular mucous membrane pemphigoid

*Correspondence, Jesse Panthagani: j.panthagani@bham.ac.ukObjectiveOcular Mucous Membrane Pemphigoid (OcMMP) is a rare disease characterised by chronic autoimmune-driven conjunctival inflammation leading to progressive scarring, and blinding sequelae. The purpose of this study was to characterise th...

Full description

Saved in:
Bibliographic Details
Published inBMJ open ophthalmology Vol. 7; no. Suppl 1; p. A1
Main Authors Panthagani, Jesse, Vincent, Rachel, Sekaran, Anisha, Pujara, Priyanka, Wallace, Graham R, Rauz, Saaeha
Format Journal Article
LanguageEnglish
Published London BMJ Publishing Group Ltd 01.05.2022
BMJ Publishing Group LTD
BMJ Publishing Group
Subjects
Online AccessGet full text

Cover

Loading…
Abstract *Correspondence, Jesse Panthagani: j.panthagani@bham.ac.ukObjectiveOcular Mucous Membrane Pemphigoid (OcMMP) is a rare disease characterised by chronic autoimmune-driven conjunctival inflammation leading to progressive scarring, and blinding sequelae. The purpose of this study was to characterise the conjunctival gene ‘fingerprint’ involved in the fibrosis signalling pathways in the pathogenesis of OcMMP.Methods and AnalysisOcular surface gene expression studies were undertaken on conjunctival swabs from OcMMP and age-matched control patients. The NanoString nCounter Human Fibrosis panel (NanoString Technologies Inc.) quantified RNA expression from 770 genes. Differentially expressed genes (DEG) and pathway analysis were determined using HyperScale architecture designed by ROSALIND, Inc. with normalisation, fold changes (≥+1.5-fold or ≤-1.5-fold) and p-values adjustment (<0.05) using the Benjamini-Hochberg method. Significantly identified genes were aligned to the aldehyde dehydrogenase (ALDH)/retinoic acid fibroblast autoregulation conjunctival scarring signalling pathway, known to be central to immune-mediated mucosal scarring in OcMMP.Results6 OcMMP patients (8 eyes, mean age 76.5 (±7.0 SD) years, 6 (66%) male, 3 (50%) biopsy-positive) and 8 age-matched cataract patients (15 eyes; age 73.1 (±9.3) years, 3 (37%) male), serving as controls were analysed. Ninety-three DEGs were observed between OcMMP and controls (48 upregulated and 45 downregulated). Of these, the top 10 upregulated DEGs were COL3A1, COL1A1, FN1, TPSAB1/B2, THBS1, SERPINE1, SPP1, COL5A1, OASL and IL1B. 44 pathways that had a global significance score greater or equal to 2, the most significant representing extracellular matrix (ECM) remodelling, synthesis, and degradation.ConclusionThe conjunctival genetic ‘fingerprint’ predominantly suggests an activated fibroblastic phenotype in the OcMMP patients and could represent (i) novel targets for drug discovery and (ii) surrogate outcomes/novel biomarkers for the monitoring of disease progression.
AbstractList *Correspondence, Jesse Panthagani: j.panthagani@bham.ac.ukOcular Mucous Membrane Pemphigoid (OcMMP) is a rare disease characterised by chronic autoimmune-driven conjunctival inflammation leading to progressive scarring, and blinding sequelae. The purpose of this study was to characterise the conjunctival gene ‘fingerprint’ involved in the fibrosis signalling pathways in the pathogenesis of OcMMP.Methods and AnalysisOcular surface gene expression studies were undertaken on conjunctival swabs from OcMMP and age-matched control patients. The NanoString nCounter Human Fibrosis panel (NanoString Technologies Inc.) quantified RNA expression from 770 genes. Differentially expressed genes (DEG) and pathway analysis were determined using HyperScale architecture designed by ROSALIND, Inc. with normalisation, fold changes (≥+1.5-fold or ≤-1.5-fold) and p-values adjustment (<0.05) using the Benjamini-Hochberg method. Significantly identified genes were aligned to the aldehyde dehydrogenase (ALDH)/retinoic acid fibroblast autoregulation conjunctival scarring signalling pathway, known to be central to immune-mediated mucosal scarring in OcMMP.Results6 OcMMP patients (8 eyes, mean age 76.5 (±7.0 SD) years, 6 (66%) male, 3 (50%) biopsy-positive) and 8 age-matched cataract patients (15 eyes; age 73.1 (±9.3) years, 3 (37%) male), serving as controls were analysed. Ninety-three DEGs were observed between OcMMP and controls (48 upregulated and 45 downregulated). Of these, the top 10 upregulated DEGs were COL3A1, COL1A1, FN1, TPSAB1/B2, THBS1, SERPINE1, SPP1, COL5A1, OASL and IL1B. 44 pathways that had a global significance score greater or equal to 2, the most significant representing extracellular matrix (ECM) remodelling, synthesis, and degradation.ConclusionThe conjunctival genetic ‘fingerprint’ predominantly suggests an activated fibroblastic phenotype in the OcMMP patients and could represent (i) novel targets for drug discovery and (ii) surrogate outcomes/novel biomarkers for the monitoring of disease progression.
*Correspondence, Jesse Panthagani: j.panthagani@bham.ac.ukObjectiveOcular Mucous Membrane Pemphigoid (OcMMP) is a rare disease characterised by chronic autoimmune-driven conjunctival inflammation leading to progressive scarring, and blinding sequelae. The purpose of this study was to characterise the conjunctival gene ‘fingerprint’ involved in the fibrosis signalling pathways in the pathogenesis of OcMMP.Methods and AnalysisOcular surface gene expression studies were undertaken on conjunctival swabs from OcMMP and age-matched control patients. The NanoString nCounter Human Fibrosis panel (NanoString Technologies Inc.) quantified RNA expression from 770 genes. Differentially expressed genes (DEG) and pathway analysis were determined using HyperScale architecture designed by ROSALIND, Inc. with normalisation, fold changes (≥+1.5-fold or ≤-1.5-fold) and p-values adjustment (<0.05) using the Benjamini-Hochberg method. Significantly identified genes were aligned to the aldehyde dehydrogenase (ALDH)/retinoic acid fibroblast autoregulation conjunctival scarring signalling pathway, known to be central to immune-mediated mucosal scarring in OcMMP.Results6 OcMMP patients (8 eyes, mean age 76.5 (±7.0 SD) years, 6 (66%) male, 3 (50%) biopsy-positive) and 8 age-matched cataract patients (15 eyes; age 73.1 (±9.3) years, 3 (37%) male), serving as controls were analysed. Ninety-three DEGs were observed between OcMMP and controls (48 upregulated and 45 downregulated). Of these, the top 10 upregulated DEGs were COL3A1, COL1A1, FN1, TPSAB1/B2, THBS1, SERPINE1, SPP1, COL5A1, OASL and IL1B. 44 pathways that had a global significance score greater or equal to 2, the most significant representing extracellular matrix (ECM) remodelling, synthesis, and degradation.ConclusionThe conjunctival genetic ‘fingerprint’ predominantly suggests an activated fibroblastic phenotype in the OcMMP patients and could represent (i) novel targets for drug discovery and (ii) surrogate outcomes/novel biomarkers for the monitoring of disease progression.
Author Sekaran, Anisha
Vincent, Rachel
Pujara, Priyanka
Wallace, Graham R
Rauz, Saaeha
Panthagani, Jesse
Author_xml – sequence: 1
  givenname: Jesse
  surname: Panthagani
  fullname: Panthagani, Jesse
  organization: Birmingham and Midland Eye Centre, Sandwell and West Birmingham Hospitals, Birmingham, UK
– sequence: 2
  givenname: Rachel
  surname: Vincent
  fullname: Vincent, Rachel
  organization: Academic Unit of Ophthalmology, Institute of Inflammation and Ageing, University of Birmingham, UK
– sequence: 3
  givenname: Anisha
  surname: Sekaran
  fullname: Sekaran, Anisha
  organization: Birmingham and Midland Eye Centre, Sandwell and West Birmingham Hospitals, Birmingham, UK
– sequence: 4
  givenname: Priyanka
  surname: Pujara
  fullname: Pujara, Priyanka
  organization: Birmingham and Midland Eye Centre, Sandwell and West Birmingham Hospitals, Birmingham, UK
– sequence: 5
  givenname: Graham R
  surname: Wallace
  fullname: Wallace, Graham R
  organization: Academic Unit of Ophthalmology, Institute of Inflammation and Ageing, University of Birmingham, UK
– sequence: 6
  givenname: Saaeha
  surname: Rauz
  fullname: Rauz, Saaeha
  organization: Birmingham and Midland Eye Centre, Sandwell and West Birmingham Hospitals, Birmingham, UK
BookMark eNpNkUtOwzAURS1UJErpCphEYpzWv9jOECo-lYrKgI4tJ3FSR4kdnASJWSfsAbbXlZBSEIze1dPVfZ9zDkbWWQ3AJYIzhAibJ3Xpmm23DTHEOLxZPM7QCRhjEvOQYBaP_ukzMG3bEkKIYsQoZmOwWT-FaL97Xzhb9jbtzKuqgkJb3Zk02O8-cmML7RtvbDeo_e4zMDZwaV8pH9R96vo2qHWdeGV10Oi62ZrCmewCnOaqavX0p07A5u72efEQrtb3y8X1KkwQj1GYZzGiGLNEiAxRJWAuIKUZ5hxyJbJUYEYwFAzGCRco5lToLCJMM5jlLOGETMDymJs5Vcphy1r5N-mUkd8N5wup_HBJpaXiPE6jKNcqSilJWJJrQqNhjKI0j_Qh6-qY1Xj30uu2k6XrvR3Wl5hxGBEsIBxc86Nr-PqfAUF5QCF_UcgDCjmgkIh8AWpKggo
ContentType Journal Article
Copyright Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
2022 Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
– notice: 2022 Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID 9YT
ACMMV
3V.
7X7
7XB
8FI
8FJ
8FK
ABUWG
AFKRA
BENPR
CCPQU
FYUFA
GHDGH
K9.
M0S
PQEST
PQQKQ
PQUKI
PRINS
DOA
DOI 10.1136/bmjophth-2022-BCM.1
DatabaseName BMJ Journals (Open Access)
BMJ Journals:Open Access
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central
ProQuest One Community College
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Health & Medical Complete (Alumni)
Health & Medical Collection (Alumni Edition)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
DOAJ Directory of Open Access Journals
DatabaseTitle ProQuest One Academic Eastern Edition
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
ProQuest Central China
ProQuest Hospital Collection (Alumni)
ProQuest Central
ProQuest Health & Medical Complete
Health Research Premium Collection
ProQuest One Academic UKI Edition
Health and Medicine Complete (Alumni Edition)
ProQuest One Academic
ProQuest Central (Alumni)
DatabaseTitleList ProQuest One Academic Eastern Edition

Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: ACMMV
  name: BMJ Journals:Open Access
  url: https://journals.bmj.com/
  sourceTypes: Publisher
– sequence: 3
  dbid: 7X7
  name: Health & Medical Collection
  url: https://search.proquest.com/healthcomplete
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EISSN 2397-3269
EndPage A1
ExternalDocumentID oai_doaj_org_article_a779c55fea5c43b6bfe3457a8a44f5e3
bmjophth
GroupedDBID 53G
5VS
7X7
8FI
8FJ
9YT
ABUWG
ACGFS
ACMMV
ADBBV
AFKRA
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BCNDV
BENPR
BTFSW
CCPQU
FYUFA
GROUPED_DOAJ
HMCUK
HYE
M~E
O9-
OK1
RHF
RHI
RMJ
RPM
UKHRP
3V.
7XB
8FK
K9.
PQEST
PQQKQ
PQUKI
PRINS
ID FETCH-LOGICAL-b1791-fd914226b88d14a80f8044d27707a8dc8263208609b7819748ed536e60df6b733
IEDL.DBID DOA
ISSN 2397-3269
IngestDate Tue Oct 22 15:09:05 EDT 2024
Thu Oct 10 17:00:10 EDT 2024
Tue Nov 26 16:40:33 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue Suppl 1
Language English
License This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ .
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-b1791-fd914226b88d14a80f8044d27707a8dc8263208609b7819748ed536e60df6b733
Notes Oral abstract presentation
2022 Proceedings of the Bowman Club Meeting, 25th March
OpenAccessLink https://doaj.org/article/a779c55fea5c43b6bfe3457a8a44f5e3
PQID 2670532800
PQPubID 5161127
ParticipantIDs doaj_primary_oai_doaj_org_article_a779c55fea5c43b6bfe3457a8a44f5e3
proquest_journals_2670532800
bmj_journals_10_1136_bmjophth_2022_BCM_1
PublicationCentury 2000
PublicationDate 20220501
PublicationDateYYYYMMDD 2022-05-01
PublicationDate_xml – month: 05
  year: 2022
  text: 20220501
  day: 01
PublicationDecade 2020
PublicationPlace London
PublicationPlace_xml – name: London
PublicationTitle BMJ open ophthalmology
PublicationTitleAbbrev BMJ Open Ophth
PublicationYear 2022
Publisher BMJ Publishing Group Ltd
BMJ Publishing Group LTD
BMJ Publishing Group
Publisher_xml – name: BMJ Publishing Group Ltd
– name: BMJ Publishing Group LTD
– name: BMJ Publishing Group
SSID ssj0001916426
Score 2.2329538
Snippet *Correspondence, Jesse Panthagani: j.panthagani@bham.ac.ukObjectiveOcular Mucous Membrane Pemphigoid (OcMMP) is a rare disease characterised by chronic...
*Correspondence, Jesse Panthagani: j.panthagani@bham.ac.ukOcular Mucous Membrane Pemphigoid (OcMMP) is a rare disease characterised by chronic...
SourceID doaj
proquest
bmj
SourceType Open Website
Aggregation Database
Publisher
StartPage A1
SubjectTerms Bowman Club 2022, 25 March 2022
Gene expression
Rare diseases
SummonAdditionalLinks – databaseName: BMJ Journals (Open Access)
  dbid: 9YT
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV27ThtBFB0RI6E0UUJAODHRFBQ0a-94nluCBUJIBgoskWo0TzCKdy1iejf8A_k9f0nujBcoaOhWU8yO7rna-9g55yJ0YJQQKkRSUGtYwRxzheHeFt5ZKas4ZIEmNvL4QpxN2PkNv2lJYYkLY2f3zfxucdeHhzWlIYk01YuBHOQxl5oMjkif9FNz-hPa5JzyNLCh-n391lmBjAfiTisxRKh43RRcAkqv49G4TyCUwGor1P_uO5yDy-lX9KXNCvHRGsZvaCPU22hr3P73_o4ml1cFWS2fRk19D4EojST7gwH6xEDEq-VzzM251KNLt5hXy394WuMmXzHFs0cH1T2ehRkUxnXA8wAATm-bqd9Bk9OT69FZ0Q5EKGxSES2irzLz1SrlCTOqjKpkzA-lLKVR3qkkvg41SllZCZFeMhU8pyKI0kdhJaW7qFM3ddhDOAjpvAtJ7F0yK7yBOq6yKkbFYM8ydtEh2Ee3Dv1X51qBCv1iSp1MqcGUmnTRcTKinq_lMXQSrM4LzcOtbv1fG0DecR6D4Y5RK2wMlHE4tWEs8kC7qPcCwdtLh0KmwRWQ0_74-HF-os8Z5nwpsYc6i4fHsA-Jw8L-yn7yHzp5xSQ
  priority: 102
  providerName: BMJ Publishing Group Ltd
– databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LTxsxELZaKlW9IGiLCC_50AMXl9211_aeEEQgVCltD0TKzfITgprdFMI9F_4D_L38EsaOoxyQuHql3dXMZ8_DM98g9ENLzqUPJaFGM8Iss0TXzhBnjRBNqJinsRt58JtfDdmvUT3KCbeHXFa5OhPTQe06G3PkJxUXcYgB-Den0_8kTo2Kt6t5hMZH9KmswJQDnsVIrHMs4PuwajlfroG9VPEmEw-VlJ-YyV03vZ3dAlAgIDvvD36WYGBgNdP3vzmdk8m53EKb2VfEZ0vlbqMPvv2KPg_ybfg3NPzzl5SL-VO_a-_APMVBZf8wACL2JeLF_DmklF3M3MXa5sX8BY9b3KXCUzx5tBDz44mfQLjcejz1oNbxTTd239Hw8uK6f0XymARiIrcoCa5J_bBGSlcyLYsgC8ZcJUQhtHRWRkp2iFyKxgiw_4JJ72rKPS9c4EZQuoM22q71uwh7LqyzPlLAC2a40xDdNUaGIBm8swg9dAzyURnmDypFEJSrlShVFKUCUaqyh86jENV0SZqhIo11Wujub1TeFUoDHmxdB69ry6jhJnjKavhrzVioPe2hg5UK1h9dI2Hv_cf76EtSbSpPPEAbs_tHfwguxMwcJZy8AlauxtA
  priority: 102
  providerName: ProQuest
Title OP-1 Conjunctival genetic ‘fingerprinting’ in ocular mucous membrane pemphigoid
URI https://bmjophth.bmj.com/content/7/Suppl_1/A1.1.full
https://www.proquest.com/docview/2670532800
https://doaj.org/article/a779c55fea5c43b6bfe3457a8a44f5e3
Volume 7
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3PT9swFLYGkxAXtA0mCqXyYYddMpLasZ1jW4EQUgBNFHUnyz-hFU0qKPde9j9s_17_Ep6doCHtwIVLIllKHH3vKe999vP3EPqmBGPC-SwhWtGEGmoSlVudWKM5L3yfOhJOI5cX7GxMzyf55FWrr1AT1sgDN8AdK3jE5Ll3KjeUaKa9IzTnSihKfe4anc-0_4pMxdUVyHog9rQyQxlhx3o-qxd3yztwC6Bfw1H5I4NwAqOtWP9__-IYYE4_oZ02M8SD5os-ow-u-oK2ynbvexeNL6-SbL36PaqrGQSj0JbsHoP5wylEvF798XGBLqzThUrm9eovnla4jmWmeP5kgOHjuZsDOa4cXjgw4vS2nto9ND49uR6dJW1ThEQHJdHE2yKeftVC2IwqkXqRUmr7nKcAijUiCLADT0kLzSHacyqczQlzLLWeaU7IV7RZ1ZXbR9gxbqxxQfCdU82sAi5XaOG9oPDO1HfQd8BHtk79KCNfIEy-QCkDlBKglFkHDQOIctFIZMggWh0HwJSyNaV8y5Qd1H0xwb9J-4yH5hWQ1x68xxyHaDs6QCxZ7KLN5cOTO4K0Yql7aINPOFyLX9c99HEwKssbuA9PLq5-9qJ3PQNHIdOK
link.rule.ids 314,780,784,864,2102,12056,21388,27924,27925,31719,33744,43310,43805,55349,55350,73745,74302,77660,77686,77707
linkProvider Directory of Open Access Journals
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LbxMxELYglYAL4inSFvCBAxfT3fVzT4hErQI0oUKN1Ju1frWpyG5o03su_Af4e_kljB1HOSBx9Uq7q5nxeL7xzDcIvWuUEMqHklDTMMIss6ThzhBnjZR1qJinsRt5PBGjKftywS9ywu02l1VufWJy1K6zMUd-VAkZhxhAfPNx8ZPEqVHxdjWP0LiP9iJzOu-hvcHx5Oz7LssC0Q-rNhPmathNlagz9VBJxZGZX3eLq-UVmApAssFw_KGEIwZWM4H_P_45HTonT9DjHC3iTxv1PkX3fPsMPRjn-_DnaPrtjJTr1a9h117DARVHlf3AYBKxMxGvV79DStrF3F2sbl6v_uBZi7tUeorndxZQP577OQDm1uOFB8XOLruZe4GmJ8fnwxHJgxKIieyiJLg6dcQapVzJGlUEVTDmKikL2ShnVSRlB-xS1EZCBCCZ8o5T4UXhgjCS0peo13atf4WwF9I66yMJvGRGuAbwXW1UCIrBO4vQR-9BPjob-q1OGIIKvRWljqLUIEpd9tEgClEvNrQZOhJZp4Xu5lLnfaEbsAjLefANt4waYYKnjMNfN4wF7mkfHW5VsPvozhb2___4LXo4Oh-f6tPPk68H6FFScypWPES95c2dfw0BxdK8yVbzF0VRyyY
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LbxMxELYglSouqLxEoMAeOHAx2V17be-pIqFReSREiEi9WX62qZrd0CT3XPgP8PfySxg7jnJA4uqVdlcznz0Pz3yD0FslGBPOF5hoRTE11GBVWY2t0ZzXvqSOhG7k0ZhdTOnny-oy1T8tU1nl_kyMB7VtTciR90rGwxAD8G96PpVFTD4OzxY_cZggFW5a0ziN--gIrGJedtBR_3w8-X7IuIAnRMvdtLkadlbJ6kRDVBDW0_ObdnG9ugbYQHjWH4zeF2BuYDWR-f9zVkcDNDxBD5PnmH3YqfoRuueax-h4lO7Gn6Dptwkutptfg7a5AWMVxpbdZgCP0KWYbTe_fUzghTxeqHTebv5ksyZrYxlqNl-bdr3M5m4OwXPjsoUDJc-u2pl9iqbD8x-DC5yGJmAdmEaxt3XsjtVC2IIqkXuRU2pLznOuhDUiELRDHJPXmoM3wKlwtiLMsdx6pjkhz1CnaRv3HGWOcWONC4TwnGpmFcR6tRbeCwrvzH0XvQP5yAT6pYzxBGFyL0oZRClBlLLoon4QolzsKDRkILWOC-3dlUx7RCpAh6kq71RlKNFMe0doBX-tKPWVI110ulfB4aMHXLz4_-M36BgAI79-Gn95iR5ELce6xVPUWd2t3SvwLVb6dQLNX4gnz1M
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=OP-1%E2%80%85Conjunctival+genetic+%E2%80%98fingerprinting%E2%80%99+in+ocular+mucous+membrane+pemphigoid&rft.jtitle=BMJ+open+ophthalmology&rft.au=Saaeha+Rauz&rft.au=Jesse+Panthagani&rft.au=Graham+R+Wallace&rft.au=Rachel+Vincent&rft.date=2022-05-01&rft.pub=BMJ+Publishing+Group&rft.eissn=2397-3269&rft.volume=7&rft.issue=Suppl+1&rft_id=info:doi/10.1136%2Fbmjophth-2022-BCM.1&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_a779c55fea5c43b6bfe3457a8a44f5e3
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2397-3269&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2397-3269&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2397-3269&client=summon