Direct Relationship between Protein Expression and Progeny Yield of Herpes Simplex Virus 1 Unveils a Rate-limiting Step for Virus Production

Although viral protein expression and progeny virus production were independently shown to be highly heterogenous in individual cells, their direct relationship, analyzed by considering their heterogeneities, has not been investigated to date. This study established a system to fractionate cells inf...

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Main Authors Nobe, Moeka, Maruzuru, Yuhei, Takeshima, Kosuke, Maeda, Fumio, Kusano, Hideo, Yoshimura, Raiki, Nishiyama, Takara, Park, Hyeongki, Kozaki, Yoshitaka, Iwami, Shingo, Koyanagi, Naoto, Kato, Akihisa, Natsume, Tohru, Adachi, Shungo, Kawaguchi, Yasushi
Format Paper
LanguageJapanese
Published Cold Spring Harbor Laboratory 08.06.2023
Edition1.2
Subjects
Microbiology
Online AccessGet full text
ISSN2692-8205
DOI10.1101/2023.06.07.544155

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Abstract Although viral protein expression and progeny virus production were independently shown to be highly heterogenous in individual cells, their direct relationship, analyzed by considering their heterogeneities, has not been investigated to date. This study established a system to fractionate cells infected with a herpesvirus based on the levels of the global expression of viral late proteins, which are largely virion structural proteins, and to titrate virus yields in these fractions. This system demonstrated a direct relationship and indicated there was a threshold for the levels of viral late protein expression for progeny virus production and suggested that viral DNA cleavage/packaging was a rate-limiting step for progeny virus production. These findings, which were masked in previous studies performed at the entire population level, have uncovered a sophisticated viral strategy for efficient progeny virus production and shed new light on an effective target for the development of anti-viral drugs.
AbstractList Although viral protein expression and progeny virus production were independently shown to be highly heterogenous in individual cells, their direct relationship, analyzed by considering their heterogeneities, has not been investigated to date. This study established a system to fractionate cells infected with a herpesvirus based on the levels of the global expression of viral late proteins, which are largely virion structural proteins, and to titrate virus yields in these fractions. This system demonstrated a direct relationship and indicated there was a threshold for the levels of viral late protein expression for progeny virus production and suggested that viral DNA cleavage/packaging was a rate-limiting step for progeny virus production. These findings, which were masked in previous studies performed at the entire population level, have uncovered a sophisticated viral strategy for efficient progeny virus production and shed new light on an effective target for the development of anti-viral drugs.
Author Kusano, Hideo
Yoshimura, Raiki
Adachi, Shungo
Kawaguchi, Yasushi
Maruzuru, Yuhei
Kozaki, Yoshitaka
Nobe, Moeka
Nishiyama, Takara
Koyanagi, Naoto
Kato, Akihisa
Iwami, Shingo
Park, Hyeongki
Maeda, Fumio
Takeshima, Kosuke
Natsume, Tohru
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Snippet Although viral protein expression and progeny virus production were independently shown to be highly heterogenous in individual cells, their direct...
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SubjectTerms Microbiology
Title Direct Relationship between Protein Expression and Progeny Yield of Herpes Simplex Virus 1 Unveils a Rate-limiting Step for Virus Production
URI https://www.biorxiv.org/content/10.1101/2023.06.07.544155
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