Dynamic design: manipulation of millisecond timescale motions on the energy landscape of Cyclophilin A

• Published in: bioRxiv
• Main Authors: Juarez-Jimenez, Jordi, Gupta, Arun A, Karunanithy, Gogulan, Mey, Antonia, Georgiou, Charis, Ioannidis, Charalampos, De Simone, Alessio, Barlow, Paul N, Hulme, Alison N, Walkinshaw, Malcolm D, Baldwin, Andrew J, Julien, Michel
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.09.2019; Cold Spring Harbor Laboratory
• Edition: 1.2
• Subjects: Biophysics; Drug development; NMR; Nuclear magnetic resonance; Protein engineering; Proteins

Proteins need to interconvert between many conformations in order to function, many of which are formed transiently, and sparsely populated. Particularly when the lifetimes of these states approach the millisecond timescale, identifying the relevant structures and the mechanism by which they inter-c...