Purification of the Recombinant Protein TmpA from Treponema pallidum Using Immobilized Metal Ion Affinity Chromatography, and Its Use in the Serodiagnosis of Syphilis
Two variants of the Treponema pallidum membrane protein A (TmpA-His, and TmpA) were expressed in Escherichia coli W3110 strain. TmpA-His was cloned with a six histidine tag at the C-terminus and was purified by immobilized metal ion affinity chromatography (IMAC). For this purpose, Sepharose 4B-IDA...
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Published in | Biotecnología aplicada Vol. 17; no. 2; pp. 89 - 93 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Cuba
Elfos Scientiae
31.12.2000
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Subjects | |
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Abstract | Two variants of the Treponema pallidum membrane protein A (TmpA-His,
and TmpA) were expressed in Escherichia coli W3110 strain. TmpA-His
was cloned with a six histidine tag at the C-terminus and was purified
by immobilized metal ion affinity chromatography (IMAC). For this
purpose, Sepharose 4B-IDA was used as matrix, and Ni 2+ and Cu2+ as
chelating metal ions. Using this rapid and single-step chromatography
the initial 20% purity of TmpA-His reached 90%. The overall yield was
70% and the binding capacity of the matrix for TmpA-His was
approximately 1 mg/mL. TmpA was purified by electroelution and the
yield was estimated to be 1 mg per each electroelution. The use of both
proteins in the diagnosis of syphilis was evaluated by ELISA using
panels of positive and negative sera. With respect to the serologic
performance no differences between both proteins were found, which
suggests the potential use of TmpA-His in the development of diagnostic
systems for syphilis serodiagnosis. |
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AbstractList | Two variants of the Treponema pallidum membrane protein A (TmpA-His,
and TmpA) were expressed in Escherichia coli W3110 strain. TmpA-His
was cloned with a six histidine tag at the C-terminus and was purified
by immobilized metal ion affinity chromatography (IMAC). For this
purpose, Sepharose 4B-IDA was used as matrix, and Ni 2+ and Cu2+ as
chelating metal ions. Using this rapid and single-step chromatography
the initial 20% purity of TmpA-His reached 90%. The overall yield was
70% and the binding capacity of the matrix for TmpA-His was
approximately 1 mg/mL. TmpA was purified by electroelution and the
yield was estimated to be 1 mg per each electroelution. The use of both
proteins in the diagnosis of syphilis was evaluated by ELISA using
panels of positive and negative sera. With respect to the serologic
performance no differences between both proteins were found, which
suggests the potential use of TmpA-His in the development of diagnostic
systems for syphilis serodiagnosis. Two variants of the Treponema pallidum membrane protein A (TmpA-His, and TmpA) were expressed in Escherichia coli W3110 strain. TmpA-His was cloned with a six histidine fag at the C-terminus and was purified by immobilized metal ion affinity chromatography (IMAC). For this purpose, Sepharose 4B-IDA was used as matrix, and Ni super(2+) and Cu super(2+) as chelating metal ions. Using this rapid and single-step chromatography the initial 20% purity of TmpA-His reached 90%. The overall yield was 70% and the binding capacity of the matrix for TmpA-His was approximately 1 mg/mL. TmpA was purified by electroelution and the yield was estimated to be 1 mg per each electroelution. The use of both proteins in the diagnosis of syphilis was evaluated by ELISA using panels of positive and negative sera. With respect to the serologic performance no differences between both proteins were found, which suggests the potential use of TmpA-His in the development of diagnostic systems for syphilis serodiagnosis. |
Author | Izquierdo, Maricela Silva, Eladio Nibot, Carmen Lubián, Ana L Tabares, Dolores Díaz, Héctor |
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Snippet | Two variants of the Treponema pallidum membrane protein A (TmpA-His,
and TmpA) were expressed in Escherichia coli W3110 strain. TmpA-His
was cloned with a six... Two variants of the Treponema pallidum membrane protein A (TmpA-His, and TmpA) were expressed in Escherichia coli W3110 strain. TmpA-His was cloned with a six... |
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SubjectTerms | affinity chromatography cromatografía de afinidad diagnosis diagnóstico IMAC protein purification syphilis sífilis TmpA TmpA protein Treponema pallidum |
Title | Purification of the Recombinant Protein TmpA from Treponema pallidum Using Immobilized Metal Ion Affinity Chromatography, and Its Use in the Serodiagnosis of Syphilis |
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