Comparison of Two Gel Electrophoresis-based DNA Micropurification Methods for Molecular Cloning

• Published in: Biotecnología aplicada Vol. 18; no. 1; pp. 29 - 31
• Main Authors: Hardy, Eugenio, Silva, Ricardo, Pupo, Elder, Hermida, Lisset, Coizeau, Edelgis, Niebla, Olivia, Castellanos-Serra, Lila
• Format: Journal Article
• Language: English
• Published: Cuba Elfos Scientiae 31.12.2001
• Subjects: bromuro de etidio; clonación; cloning; electroforesis en gel; ethidium bromide; gel electrophoresis; luz ultravioleta; polyacrylamide; tinción con zinc; ultraviolet-light; zinc staining

We recently described a method for recovering nucleic acids separated from polyacrylamide gels, which is based on the sensitive on-gel DNA detection with zinc-imidazole followed by passive elution from 32-mm average-size gel microparticles into the elution buffer. The rationale for the preferential...