3D Adaptive Optical Nanoscopy for Thick Specimen Imaging at sub-50 nm Resolution

• Published in: bioRxiv
• Main Authors: Xiang Hao, Allgeyer, Edward S, Antonello, Jacopo, Watters, Katherine, Gerdes, Julianne A, Schroeder, Lena K, Bottanelli, Francesca, Zhao, Jiaxi, Kidd, Phylicia, Lessard, Mark D, Rothman, James E, Cooley, Lynn, Biederer, Thomas, Booth, Martin J, Bewersdorf, Joerg
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 27.11.2020; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Biophysics; Diffraction; Fluorescence microscopy; Microscopes; Optics; Spatial discrimination

Understanding cellular organization demands the best possible spatial resolution in all three dimensions (3D). In fluorescence microscopy, this is achieved by 4Pi nanoscopy methods that combine the concepts of using two opposing objectives for optimal diffraction-limited 3D resolution with switching...