Enhanced virus detection and metagenomic sequencing in patients with meningitis and encephalitis

• Published in: bioRxiv
• Main Authors: Piantadosi, Anne, Mukerji, Shibani S, Ye, Simon, Leone, Michael J, Freimark, Lisa M, Park, Daniel, Adams, Gordon, Lemieux, Jacob, Kanjilal, Sanjat, Solomon, Isaac H, Ahmed, Asim A, Goldstein, Robert, Ganesh, Vijay, Ostrem, Bridget, Cummins, Kaelyn C, Thon, Jesse M, Kinsella, Cormac M, Rosenberg, Eric, Frosch, Matthew P, Goldberg, Marcia B, Cho, Tracey A, Sabeti, Pardis
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 27.11.2020; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Central nervous system; Cerebrospinal fluid; Computational neuroscience; Deoxyribonucleic acid; DNA; DNA viruses; Encephalitis; Enteroviruses; Epstein-Barr virus; Laboratories; Meningitis; Metagenomics; Nervous system; Neuroscience; Next-generation sequencing; Pathogens; Patients; Serology; Varicella; metagenomic sequencing; meningitis, virus; methylated DNA depletion; hybrid capture; encephalitis; next-generation sequencing (NGS)

Abstract Meningitis and encephalitis are leading causes of central nervous system (CNS) disease and often result in severe neurological compromise or death. Traditional diagnostic workflows largely rely on pathogen-specific diagnostic tests, sometimes over days to weeks. Metagenomic next-generation...