The integrated stress response induces R-loops and hinders replication fork progression

• Published in: bioRxiv
• Main Authors: Josephine Ann Mun Yee Choo, Schloesser, Denise, Manzini, Valentina, Magerhans, Anna, Dobbelstein, Matthias
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 11.03.2020; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Amino acids; Cancer Biology; Deoxyribonucleic acid; DNA; DNA biosynthesis; Hybrids; Phosphorylation; Protein biosynthesis; Protein deficiency; Protein folding; Protein synthesis; Proteins; R-loops; Replication forks; Ribonucleic acid; RNA; Shutdowns; Translation; GCN2; Thapsigargin; DNA replication; histones; Integrated stress response; PKR; ISRIB; PERK; eIF2alpha; R-loops; BEPP; DNA fiber assays

The integrated stress response (ISR) allows cells to rapidly shut down most of their protein synthesis in response to protein misfolding, amino acid deficiency, or virus infection. These stresses trigger the phosphorylation of the translation initiation factor eIF2alpha, which prevents the initiatio...