All-optical visualization of specific molecules in the ultrastructural context of brain tissue

• Published in: bioRxiv
• Main Authors: Ons M'saad, Kasula, Ravikiran, Kondratiuk, Ilona, Kidd, Phylicia, Falahati, Hanieh, Gentile, Juliana E, Niescier, Robert F, Watters, Katherine, Sterner, Robert C, Lee, Seong, Liu, Xinran, De Camilli, Pietro, Rothman, James E, Koleske, Anthony J, Biederer, Thomas, Bewersdorf, Joerg
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.04.2022; Cold Spring Harbor Laboratory
• Edition: 1.2
• Subjects: Bassoon protein; Electron microscopy; Glial fibrillary acidic protein; Microscopy; Myelin; Myelin basic protein; Neural networks; Neuroimaging; Neuropil; Neuroscience; Organelles; Patent applications; Postsynaptic density proteins; Protein folding; Proteins; Synaptophysin; Ultrastructure

Understanding the molecular anatomy and neural connectivity of the brain requires imaging technologies that can map the 3D nanoscale distribution of specific proteins in the context of brain ultrastructure. Light and electron microscopy (EM) enable visualization of either specific labels or anatomic...