Methodology development and evaluation of optical properties characterisation of small size tissue samples
Integrating sphere (IS) techniques combined with an inverse adding doubling (IAD) algorithm have been widely used for determination of optical properties of ex vivo tissues. Semi-infinite samples are required in such cases. The aim of this study is to develop a methodology for calculating the optica...
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Main Authors | , , , , , , |
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Format | Journal Article |
Language | English |
Published |
10.07.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Integrating sphere (IS) techniques combined with an inverse adding doubling
(IAD) algorithm have been widely used for determination of optical properties
of ex vivo tissues. Semi-infinite samples are required in such cases. The aim
of this study is to develop a methodology for calculating the optical
absorption and reduced scattering of biological tissues of small size from IS
measurements at 400-1800 nm. We propose a two-stage IAD algorithm to mitigate
profound cross-talk effects in the estimation of the reduced scattering in the
case of very high absorption. We developed a small sample adaptor kit to allow
IS measurements of samples with small sizes using a commercial
spectrophotometer. Results showed that: The two-stage IAD substantially
eliminated the cross-talks in the reduced scattering spectra, thus rectifying
absorption accordingly; and the small sized sample measurements led to
systematically overestimated absorption values while the spectrum shape well
preserved as compared to the normal port size measurements. |
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DOI: | 10.48550/arxiv.2107.04909 |