Nellie: Automated organelle segmentation, tracking, and hierarchical feature extraction in 2D/3D live-cell microscopy

• Main Authors: Lefebvre, Austin E. Y. T, Sturm, Gabriel, Lin, Ting-Yu, Stoops, Emily, Lopez, Magdalena Preciado, Kaufmann-Malaga, Benjamin, Hake, Kayley
• Format: Journal Article
• Language: English
• Published: 19.03.2024
• Subjects: Computer Science - Artificial Intelligence; Computer Science - Computer Vision and Pattern Recognition; Computer Science - Learning; Quantitative Biology - Quantitative Methods

The analysis of dynamic organelles remains a formidable challenge, though key to understanding biological processes. We introduce Nellie, an automated and unbiased pipeline for segmentation, tracking, and feature extraction of diverse intracellular structures. Nellie adapts to image metadata, elimin...