Mass Spectrometric Identification of SARS-CoV‑2 Proteins from Gargle Solution Samples of COVID-19 Patients

Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solutio...

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Published inJournal of proteome research Vol. 19; no. 11; pp. 4389 - 4392
Main Authors Ihling, Christian, Tänzler, Dirk, Hagemann, Sven, Kehlen, Astrid, Hüttelmaier, Stefan, Arlt, Christian, Sinz, Andrea
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 06.11.2020
Subjects
acetone
Betacoronavirus - chemistry
Chromatography, High Pressure Liquid
Clinical Laboratory Techniques
Communication
Coronavirus Infections - diagnosis
Coronavirus Infections - virology
COVID-19
COVID-19 infection
COVID-19 Testing
digestion
genomics
Humans
mass spectrometry
Mass Spectrometry - methods
Mouth - virology
nucleoproteins
Nucleoproteins - analysis
Nucleoproteins - chemistry
Pandemics
Peptide Fragments - analysis
Peptide Fragments - chemistry
peptides
Pneumonia, Viral - diagnosis
Pneumonia, Viral - virology
proteome
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Viral Proteins - analysis
Viral Proteins - chemistry
viruses
COVID-19
nucleoprotein
mass spectrometry
SARS-CoV-2
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Abstract Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.
AbstractList Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.
Author Hagemann, Sven
Arlt, Christian
Kehlen, Astrid
Tänzler, Dirk
Hüttelmaier, Stefan
Sinz, Andrea
Ihling, Christian
AuthorAffiliation Department of Molecular Cell Biology, Institute for Molecular Medicine, Charles Tanford Center, Medical Faculty
Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy
Martin Luther University Halle-Wittenberg
Institute of Medicinal Microbiology, Biocenter, Medical Faculty
AuthorAffiliation_xml – name: Martin Luther University Halle-Wittenberg
– name: Department of Molecular Cell Biology, Institute for Molecular Medicine, Charles Tanford Center, Medical Faculty
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  organization: Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy
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Cites_doi 10.1093/nar/gky1106
10.1101/2020.03.12.988865
10.1016/S2213-2600(20)30079-5
10.1016/S0140-6736(20)31211-3
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Keywords COVID-19
nucleoprotein
mass spectrometry
SARS-CoV-2
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Snippet Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19...
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19...
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SubjectTerms acetone
Betacoronavirus - chemistry
Chromatography, High Pressure Liquid
Clinical Laboratory Techniques
Communication
Coronavirus Infections - diagnosis
Coronavirus Infections - virology
COVID-19
COVID-19 infection
COVID-19 Testing
digestion
genomics
Humans
mass spectrometry
Mass Spectrometry - methods
Mouth - virology
nucleoproteins
Nucleoproteins - analysis
Nucleoproteins - chemistry
Pandemics
Peptide Fragments - analysis
Peptide Fragments - chemistry
peptides
Pneumonia, Viral - diagnosis
Pneumonia, Viral - virology
proteome
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Viral Proteins - analysis
Viral Proteins - chemistry
viruses
Title Mass Spectrometric Identification of SARS-CoV‑2 Proteins from Gargle Solution Samples of COVID-19 Patients
URI http://dx.doi.org/10.1021/acs.jproteome.0c00280
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