Structure and DNA Hybridization Properties of Mixed Nucleic Acid/Maleimide−Ethylene Glycol Monolayers
The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide−ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied...
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| Published in | Analytical chemistry (Washington) Vol. 79; no. 12; pp. 4390 - 4400 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Chemical Society
15.06.2007
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide−ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. Orientation of the ssDNA probes was determined by near-edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA−MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the N 1s → π* transition) indicate that the immobilized ssDNA molecules reorient toward a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the “high-density” probe surface than on the “high-efficiency” probe surface. The amounts of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to nonspecific serum protein adsorption onto the sensing surface. |
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| AbstractList | The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide-ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. Orientation of the ssDNA probes was determined by near-edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA-MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the ... transition) indicate that the immobilized ssDNA molecules reorient toward a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the "high-density" probe surface than on the "high-efficiency" probe surface. The amounts of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to nonspecific serum protein adsorption onto the sensing surface. (ProQuest-CSA LLC: ... denotes formulae/symbols omitted.) The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide-ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. Orientation of the ssDNA probes was determined by near-edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA-MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the N 1s --> pi* transition) indicate that the immobilized ssDNA molecules reorient toward a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the "high-density" probe surface than on the "high-efficiency" probe surface. The amounts of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to nonspecific serum protein adsorption onto the sensing surface. The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide-ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Orientation of the ssDNA probes was determined by near edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA-MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the N 1s → π * transition) indicate that the immobilized ssDNA molecules reorient towards a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the “high density” probe surface than on the “high efficiency” probe surface. The amount of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to non-specific serum protein adsorption onto the sensing surface. The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide-ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. Orientation of the ssDNA probes was determined by near-edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA-MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the N 1s {yields} {pi}* transition) indicate that the immobilized ssDNA molecules reorient toward a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the 'high-density' probe surface than on the 'high-efficiency' probe surface. The amounts of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to nonspecific serum protein adsorption onto the sensing surface. The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide-ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. Orientation of the ssDNA probes was determined by near-edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA-MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the N Is arrow right capital pi * transition) indicate that the immobilized ssDNA molecules reorient toward a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the "high-density" probe surface than on the "high-efficiency" probe surface. The amounts of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to nonspecific serum protein adsorption onto the sensing surface. The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide−ethylene glycol disulfide (MEG) monolayer on gold have been investigated. Monolayer immobilization chemistry and surface coverage of reactive ssDNA probes were studied by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. Orientation of the ssDNA probes was determined by near-edge X-ray absorption fine structure (NEXAFS). Target DNA hybridization on the DNA−MEG probe surfaces was measured by surface plasmon resonance (SPR) to demonstrate the utility of these probe surfaces for detection of DNA targets from both purified target DNA samples and complex biological mixtures such as blood serum. Data from complementary techniques showed that immobilized ssDNA density is strongly dependent on the spotted bulk DNA concentration and buffer ionic strength. Variation of the immobilized ssDNA density had a profound influence on the DNA probe orientation at the surface and subsequent target hybridization efficiency. With increasing surface probe density, NEXAFS polarization dependence results (followed by monitoring the N 1s → π* transition) indicate that the immobilized ssDNA molecules reorient toward a more upright position on the MEG monolayer. SPR assays of DNA targets from buffer and serum showed that DNA hybridization efficiency increased with decreasing surface probe density. However, target detection in serum was better on the “high-density” probe surface than on the “high-efficiency” probe surface. The amounts of target detected for both ssDNA surfaces were several orders of magnitude poorer in serum than in purified DNA samples due to nonspecific serum protein adsorption onto the sensing surface. |
| Author | Castner, David G Gamble, Lara J Lee, Chi-Ying Nguyen, Phuong-Cac T Grainger, David W |
| AuthorAffiliation | 3 Departments of Pharmaceutics, University of Utah, Salt Lake City, UT 84112-5820 2 Departments of Bioengineering and Chemical Engineering, Box 351750 University of Washington, Seattle, WA 98195-1750 4 Departments of Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112-5820 5 Departments of Bioengineering, University of Utah, Salt Lake City, UT 84112-5820 1 National ESCA and Surface Analysis Center for Biomedical Problems, Box 351750 University of Washington, Seattle, WA 98195-1750 |
| AuthorAffiliation_xml | – name: 1 National ESCA and Surface Analysis Center for Biomedical Problems, Box 351750 University of Washington, Seattle, WA 98195-1750 – name: 4 Departments of Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112-5820 – name: 2 Departments of Bioengineering and Chemical Engineering, Box 351750 University of Washington, Seattle, WA 98195-1750 – name: 5 Departments of Bioengineering, University of Utah, Salt Lake City, UT 84112-5820 – name: 3 Departments of Pharmaceutics, University of Utah, Salt Lake City, UT 84112-5820 |
| Author_xml | – sequence: 1 givenname: Chi-Ying surname: Lee fullname: Lee, Chi-Ying – sequence: 2 givenname: Phuong-Cac T surname: Nguyen fullname: Nguyen, Phuong-Cac T – sequence: 3 givenname: David W surname: Grainger fullname: Grainger, David W – sequence: 4 givenname: Lara J surname: Gamble fullname: Gamble, Lara J – sequence: 5 givenname: David G surname: Castner fullname: Castner, David G |
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| Keywords | Performance evaluation Monolayer Ethylene glycol Gold Chemical analysis Immobilization X ray spectrometry Secondary ion mass spectrometry Protein Blood Chemical enrichment Surface structure Biological compound Adsorption Efficiency Ionic strength Time of flight method DNA Serum Surface plasmon resonance DNA hybridization Nucleic acid Monitoring |
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| Snippet | The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide−ethylene glycol disulfide... The surface structure and DNA hybridization performance of thiolated single-strand DNA (HS-ssDNA) covalently attached to a maleimide-ethylene glycol disulfide... |
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| SubjectTerms | ABSORPTION ADSORPTION Analytical chemistry BASIC BIOLOGICAL SCIENCES BLOOD SERUM BUFFERS CHEMISTRY Deoxyribonucleic acid DETECTION DISULFIDES DNA DNA - analysis DNA - chemistry DNA HYBRIDIZATION DNA Probes DNA, Single-Stranded - chemistry EFFICIENCY Ethylene Glycol - chemistry Exact sciences and technology FINE STRUCTURE GLYCOLS GOLD Gold - chemistry HYBRIDIZATION Maleimides - chemistry Mass spectrometry MASS SPECTROSCOPY MIXTURES MONITORING national synchrotron light source Nucleic Acid Hybridization ORIENTATION Osmolar Concentration PHYSICS OF ELEMENTARY PARTICLES AND FIELDS PLASMONS POLARIZATION PROBES PROTEINS RESONANCE Spectrometric and optical methods Spectrometry, Mass, Secondary Ion Spectrum Analysis Sulfhydryl Compounds - chemistry Surface chemistry Surface Properties Temperature Time Factors X-RAY PHOTOELECTRON SPECTROSCOPY X-Rays |
| Title | Structure and DNA Hybridization Properties of Mixed Nucleic Acid/Maleimide−Ethylene Glycol Monolayers |
| URI | http://dx.doi.org/10.1021/ac0703395 https://api.istex.fr/ark:/67375/TPS-BTJTSB1P-0/fulltext.pdf https://www.ncbi.nlm.nih.gov/pubmed/17492838 https://www.proquest.com/docview/217880782 https://search.proquest.com/docview/20360631 https://search.proquest.com/docview/70620646 https://www.osti.gov/biblio/930464 https://pubmed.ncbi.nlm.nih.gov/PMC2518630 |
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