Tracking Single Lipase Molecules on a Trimyristin Substrate Surface Using Quantum Dots

• Published in: Langmuir Vol. 23; no. 16; pp. 8352 - 8356
• Main Authors: Sonesson, Andreas W, Elofsson, Ulla M, Callisen, Thomas H, Brismar, Hjalmar
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 31.07.2007
• Subjects: Binding Sites; Biological detection; Chemistry; Colloidal state and disperse state; Diffusion; Domains; Dynamics; Enzyme-kinetics; Exact sciences and technology; Fysik; General and physical chemistry; Humicola-languginosa lipase; humicola-lanuginosa lipase; Hydrolysis; Interfacial activation; Lipase - chemistry; Medicin och hälsovetenskap; Membrane; Mobility; NATURAL SCIENCES; NATURVETENSKAP; Physics; Quantum Dots; Receptors; Surface physical chemistry; Triglycerides - chemistry; Quantum dot; Tracking; Spin; Active site; Mobility; Confocal microscopy; Displacement; Substrate; Imaging; Laser; Trajectory; Diffusion coefficient; Diffusion

The mobility of single lipase molecules has been analyzed using single molecule tracking on a trimyristin substrate surface. This was achieved by conjugating lipases to quantum dots and imaging on spin-coated trimyristin surfaces by means of confocal laser scanning microscopy. Image series of single...