Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078

• Published in: mSystems Vol. 5; no. 3
• Main Authors: Li, Tinghua, Zhang, Yan, Dong, Ke, Kuo, Chih-Jung, Li, Chong, Zhu, Yong-Qiang, Qin, Jinhong, Li, Qing-Tian, Chang, Yung-Fu, Guo, Xiaokui, Zhu, Yongzhang
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 05.05.2020
• Subjects: Antibiotics; Bacteria; bacteria-phage interaction; bacteriophage; Clostridioides difficile; CRISPR; Deoxyribonucleic acid; Diarrhea; DNA; DNA biosynthesis; Drug resistance; Gene expression; Genomes; Infections; Intestine; Lysis; Lysogeny; Macromolecules; Metabolism; Microbiota; Mortality; Phages; Phenotypes; Phylogenetics; Predation; Prophages; Research Article; Restriction-modification; ribotype 078; RNA-seq; Spore-forming bacteria; Sporulation; Therapeutics and Prevention; Transcription; transcriptome; Transcriptomics; Virulence factors; Clostridioides difficile; bacteria-phage interaction; RNA-seq; ribotype 078; bacteriophage; transcriptome

C. difficile is one of the most clinically significant intestinal pathogens. Although phages have been shown to effectively control C. difficile infection, the host responses to phage predation have not been fully studied. In this study, we reported the isolation and characterization of a new phage,...