Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes

• Published in: ACS Omega Vol. 6; no. 11; pp. 7374 - 7386
• Main Authors: Takahashi, Masateru, Tehseen, Muhammad, Salunke, Rahul, Takahashi, Etsuko, Mfarrej, Sara, Sobhy, Mohamed A, Alhamlan, Fatimah S, Hala, Sharif, Ramos-Mandujano, Gerardo, Al-Qahtani, Ahmed A, Alofi, Fadwa S, Alsomali, Afrah, Hashem, Anwar M, Khogeer, Asim, Almontashiri, Naif A. M, Lee, Jae Man, Mon, Hiroaki, Sakashita, Kosuke, Li, Mo, Kusakabe, Takahiro, Pain, Arnab, Hamdan, Samir M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 23.03.2021; American Chemical Society (ACS)
• Subjects: 3T; Biochemistry; Biophysics; Biotechnology; Chemistry; Computational Biology; COVID -19; COVID -19 Diagnostics; Developmental Biology; enzyme production strategies; Genetics; Health-approved testing facility; Immunology; In-House Enzymes One-step reverse-t; Infectious Diseases; Invitrogen SuperScript III Platinum; Molecular Biology; Moloney murine leukemia virus; One-Step qRT-PCR Kit; polymerase chain reaction; QD1-999; Rapid Research Response Team; RNA; Thermus aquaticus DNA polymerase; Virology

One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding the facts that one-step qRT-PCR is well suited for the diagnosis of COVID-19 and that there are many commercially available one-step qRT-PCR kit...