Identification of the High Mannose N‑Glycan Isomers Undescribed by Conventional Multicellular Eukaryotic Biosynthetic Pathways

• Published in: Analytical chemistry (Washington) Vol. 95; no. 23; pp. 8789 - 8797
• Main Authors: Liew, Chia Yen, Luo, Hong-Sheng, Yang, Ting-Yi, Hung, An-Ti, Magoling, Bryan John Abel, Lai, Charles Pin-Kuang, Ni, Chi-Kung
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 13.06.2023
• Subjects: analytical chemistry; Animalia; Biosynthesis; Biosynthetic Pathways; Chemistry; Endoplasmic reticulum; Eukaryota - metabolism; Eukaryotes; eukaryotic cells; Glycan; Glycosylation; Golgi apparatus; Humans; Ions; Isomers; Mannose; Mannose - chemistry; Mass spectra; Mass spectrometry; Mass spectroscopy; Nucleotide sequence; Polysaccharides; Polysaccharides - chemistry; Post-translation; Retention time; Scientific imaging; tandem mass spectrometry; Tandem Mass Spectrometry - methods

N-linked glycosylation is one of the most important post-translational modifications of proteins. Current knowledge of multicellular eukaryote N-glycan biosynthesis suggests high mannose N-glycans are generated in the endoplasmic reticulum and Golgi apparatus through conserved biosynthetic pathways....