Site-Specific Bioconjugation through Enzyme-Catalyzed Tyrosine–Cysteine Bond Formation

• Published in: ACS central science Vol. 6; no. 9; pp. 1564 - 1571
• Main Authors: Lobba, Marco J, Fellmann, Christof, Marmelstein, Alan M, Maza, Johnathan C, Kissman, Elijah N, Robinson, Stephanie A, Staahl, Brett T, Urnes, Cole, Lew, Rachel J, Mogilevsky, Casey S, Doudna, Jennifer A, Francis, Matthew B
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 23.09.2020

The synthesis of protein–protein and protein–peptide conjugates is an important capability for producing vaccines, immunotherapeutics, and targeted delivery agents. Herein we show that the enzyme tyrosinase is capable of oxidizing exposed tyrosine residues into o-quinones that react rapidly with cys...