Considerations for the Analysis of Bacterial Membrane Vesicles: Methods of Vesicle Production and Quantification Can Influence Biological and Experimental Outcomes

• Published in: Microbiology spectrum Vol. 9; no. 3; p. e0127321
• Main Authors: Bitto, Natalie J, Zavan, Lauren, Johnston, Ella L, Stinear, Timothy P, Hill, Andrew F, Kaparakis-Liaskos, Maria
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 22.12.2021
• Subjects: bacterial membrane vesicles (BMVs); Bacterial Outer Membrane - metabolism; Bacterial Proteins - analysis; Cell Line; DNA; Extracellular Vesicles - metabolism; HEK293 Cells; Helicobacter pylori - genetics; Helicobacter pylori - metabolism; Host Microbial Interactions; Humans; membrane vesicles (MVs); NTA; outer membrane vesicles (OMVs); Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - metabolism; Research Article; RNA; RNA, Bacterial - genetics; Staphylococcus aureus - genetics; Staphylococcus aureus - metabolism; quantification; RNA; outer membrane vesicles (OMVs); membrane vesicles (MVs); DNA; protein; NTA; bacterial membrane vesicles (BMVs); TLRs

Bacterial membrane vesicles (BMVs) are produced by all bacteria and facilitate a range of functions in host-microbe interactions and pathogenesis. Quantification of BMVs is a critical first step in the analysis of their biological and immunological functions. Historically, BMVs have been quantified...