MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model

• Published in: Analytical chemistry (Washington) Vol. 87; no. 12; pp. 5947 - 5956
• Main Authors: Jiang, Lu, Chughtai, Kamila, Purvine, Samuel O, Bhujwalla, Zaver M, Raman, Venu, Paša-Tolić, Ljiljana, Heeren, Ron M. A, Glunde, Kristine
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.06.2015; American Chemical Society (ACS)
• Subjects: 60 APPLIED LIFE SCIENCES; Animals; Breast; breast cancer; Cell Hypoxia; Cell Line, Tumor; Cellular biology; Environmental Molecular Sciences Laboratory; Female; Humans; Hypoxia; hypoxia, phosphatidylcholine; Imaging; lipid, peptide; Lipids; Lipids - analysis; Mammary Neoplasms, Experimental - chemistry; mass spectrometric imaging; Mass spectrometry; Mice; Neoplasm Proteins - analysis; Pathways; protein; Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stress response; Three dimensional; Tumors

Hypoxic areas are a common feature of rapidly growing malignant tumors and their metastases and are typically spatially heterogeneous. Hypoxia has a strong impact on tumor cell biology and contributes to tumor progression in multiple ways. To date, only a few molecular key players in tumor hypoxia,...