Separation of Isomeric O-Glycans by Ion Mobility and Liquid Chromatography–Mass Spectrometry

• Published in: Analytical chemistry (Washington) Vol. 91; no. 16; pp. 10604 - 10613
• Main Authors: Jin, Chunsheng, Harvey, David J, Struwe, Weston B, Karlsson, Niclas G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.08.2019
• Subjects: Analytical Chemistry; Analytisk kemi; Biological properties; Biological samples; Biosynthesis; blood-group; carbon; Cellular structure; Chemistry; Chromatography; collision cross-sections; fragmentation; gases; genome; Genomes; Glycan; Glycoconjugates; Glycosidases; Glycosylation; glycosyltransferases; Graphitization; humans; Ionic mobility; Ions; Isomers; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Mobility; Mucin; Mucins; N-glycans; negative-ions; Oligosaccharides; Phase separation; Polysaccharides; Post-translation; Scientific imaging; Sensitivity analysis; spectrometers; Spectroscopy; Structural analysis; structural determination; swine; unicarb-db

Glycosylation is one of the most important post-translational modifications essential for modulating biological functions on cellular surfaces and within cells. Glycan structures are not predictable from the genome since their biosynthesis is nontemplate driven and subject to multiple sequential and...