Characterization and Sequence Mapping of Large RNA and mRNA Therapeutics Using Mass Spectrometry

• Published in: Analytical chemistry (Washington) Vol. 94; no. 20; pp. 7339 - 7349
• Main Authors: Vanhinsbergh, Christina J., Criscuolo, Angela, Sutton, Jennifer N., Murphy, Keeley, Williamson, Andrew J. K., Cook, Ken, Dickman, Mark J.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 24.05.2022
• Subjects: Analytical chemistry; Analytical methods; Annotations; Automation; Chemistry; Gene sequencing; Liquid chromatography; Mapping; Mass spectrometry; Mass spectroscopy; mRNA; Nucleotide sequence; Proteins; Ribonuclease T1; Sample preparation; Scientific imaging; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spectroscopy; Spike protein; Vaccines; Workflow

Large RNA including mRNA (mRNA) has emerged as an important new class of therapeutics. Recently, this has been demonstrated by two highly efficacious vaccines based on mRNA sequences encoding for a modified version of the SARS-CoV-2 spike protein. There is currently significant demand for the develo...