Dynamic Assessment of Bilayer Thickness by Varying Phospholipid and Hydraphile Synthetic Channel Chain Lengths

• Published in: Journal of the American Chemical Society Vol. 127; no. 2; pp. 636 - 642
• Main Authors: Weber, Michelle E, Schlesinger, Paul H, Gokel, George W
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 19.01.2005; Amer Chemical Soc
• Subjects: Artificial membranes and reconstituted systems; Biological and medical sciences; Chemistry; Chemistry, Multidisciplinary; Dimyristoylphosphatidylcholine - chemistry; Fundamental and applied biological sciences. Psychology; Ion Channels - chemistry; Ion Channels - metabolism; Lipid Bilayers - chemistry; Liposomes - chemistry; Membrane physicochemistry; Molecular biophysics; Nuclear Magnetic Resonance, Biomolecular; Phosphatidylcholines - chemistry; Phospholipids - chemistry; Phosphorylcholine - analogs & derivatives; Phosphorylcholine - chemistry; Physical Sciences; Science & Technology; Sodium - chemistry; Sodium - metabolism; Sodium Channels - chemistry; Sodium Channels - metabolism; SODIUM; LIPID-BILAYERS; CHOLESTEROL; FLUORESCENCE; EQUILIBRIA; MEMBRANE; X-RAY; MODEL; ANGLE NEUTRON-SCATTERING; MODULATION; Ion selective electrode; Structure effect; Electrochemical method; Chain length; Phospholipid; Ion transport; Artificial membrane; Bilayer

A library of “hydraphile” synthetic ion channel analogues that differ in overall length from ∼28−58 Å has been prepared. A new and convenient ion-selective electrode (ISE) method was used to assay Na+ release. Liposomes were formed from three different phospholipids:  1,2-dimyristoleoyl-sn-glycero-3...