Systematic Evaluation of the Viable Microbiome in the Human Oral and Gut Samples with Spike-in Gram+/– Bacteria

• Published in: mSystems Vol. 8; no. 2; p. e0073822
• Main Authors: Liu, Feng, Lu, Hui, Dong, Biao, Huang, Xiaochang, Cheng, Hongyu, Qu, Ru, Hu, Yichen, Zhong, Luyun, Guo, Zhenni, You, Yuehua, Xu, Zhenjiang Zech
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 27.04.2023
• Subjects: Bacteria; Bacteria - genetics; Community composition; DNA; DNA, Bacterial - genetics; E coli; Efficiency; Feces; Feces - microbiology; Firmicutes; Firmicutes - genetics; Freezing; Glycerol; host depletion; Human Microbiome; Humans; live/dead bacteria; Lysis; Metagenomics; Microbiomes; Microbiota; Microbiota - genetics; Microorganisms; Nucleotide sequence; Phenotypes; PMAxx; Relative abundance; Research Article; Saliva; Strains (organisms); metagenomic sequencing; saliva; freezing; live/dead bacteria; host depletion; feces; PMAxx

The functions and phenotypes of microbial communities are largely defined by viable microbes. Through advanced nucleic acid sequencing technologies and downstream bioinformatic analyses, we gained an insight into the high-resolution microbial community composition of human saliva and feces, yet we k...