Metabolic Profile of the Genome-Reduced Bacillus subtilis Strain IIG-Bs-27-39: An Attractive Chassis for Recombinant Protein Production

• Published in: ACS Synthetic Biology Vol. 13; no. 7; pp. 2199 - 2214
• Main Authors: Aguilar Suárez, Rocío, Kohlstedt, Michael, Öktem, Ayşegül, Neef, Jolanda, Wu, Yuzheng, Ikeda, Kaiya, Yoshida, Ken-Ichi, Altenbuchner, Josef, Wittmann, Christoph, van Dijl, Jan Maarten
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.07.2024; American Chemical Society (ACS)
• Subjects: Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bioreactors; energetic parameters; genome reduction; Genome, Bacterial - genetics; Metabolic Engineering - methods; metabolic features; Metabolome; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; energetic parameters; genome reduction; metabolic features; Bacillus subtilis

The Gram-positive bacterium Bacillus subtilis is extensively used in the industry for the secretory production of proteins with commercial value. To further improve its performance, this microbe has been the subject of extensive genome engineering efforts, especially the removal of large genomic reg...