Simultaneous Detection of Multiple DNA Adducts in Human Lung Samples by Isotope-Dilution UPLC-MS/MS

• Published in: Analytical chemistry (Washington) Vol. 87; no. 1; pp. 641 - 648
• Main Authors: Monien, Bernhard H, Schumacher, Fabian, Herrmann, Kristin, Glatt, Hansruedi, Turesky, Robert J, Chesné, Christophe
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.01.2015
• Subjects: Adducts; Analytical chemistry; benzo(a)pyrene; biomarkers; biopsy; Chromatography; Chromatography, Liquid - methods; Deoxyribonucleic acid; detection limit; Dilution; DNA; DNA adducts; DNA Adducts - analysis; DNA Adducts - chemistry; furfuryl alcohol; Human; Humans; hydrolysates; hydroxymethylfurfural; Indicator Dilution Techniques; Isotopes; lipid peroxidation; Liquid chromatography; Lung - metabolism; Lungs; malondialdehyde; Mass spectrometry; mutagenicity; Mutagens; Nucleosides; Peroxidation; pyridines; Risk assessment; Spectroscopy; tandem mass spectrometry; Tandem Mass Spectrometry - methods; tissues; ultra-performance liquid chromatography

Recent studies have demonstrated that various DNA adducts can be detected in human tissues and fluids using liquid chromatography connected to tandem mass spectrometry (LC-MS/MS). However, the utility of a single DNA adduct as a biomarker in risk assessment is debatable because humans are exposed to...