Measurement of DNA Concentration as a Normalization Strategy for Metabolomic Data from Adherent Cell Lines

• Published in: Analytical chemistry (Washington) Vol. 85; no. 20; pp. 9536 - 9542
• Main Authors: Silva, Leslie P., Lorenzi, Philip L., Purwaha, Preeti, Yong, Valeda, Hawke, David H., Weinstein, John N.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.10.2013
• Subjects: Biochemistry; Biotechnology; Bulging; Cell Adhesion; Cell adhesion & migration; Cell Count; Cell Line, Tumor; Clumps; Consistency; Correlation; Correlation analysis; Counting; Deoxyribonucleic acid; DNA; DNA - analysis; Extraction processes; Humans; Linear Models; Metabolites; metabolomics; Metabolomics - methods; protein content; Strategy; Time Factors

Metabolomics is a rapidly advancing field, and much of our understanding of the subject has come from research on cell lines. However, the results and interpretation of such studies depend on appropriate normalization of the data; ineffective or poorly chosen normalization methods can lead to frankl...