Biostable L‑DNAzyme for Sensing of Metal Ions in Biological Systems

• Published in: Analytical chemistry (Washington) Vol. 88; no. 3; pp. 1850 - 1855
• Main Authors: Cui, Liang, Peng, Ruizi, Fu, Ting, Zhang, Xiaobing, Wu, Cuichen, Chen, Huapei, Liang, Hao, Yang, Chaoyong James, Tan, Weihong
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.02.2016
• Subjects: Binding; Biological; Biological samples; Biosensors; Catalysis; Cleavage; Copper - analysis; Copper - metabolism; Deoxyribonucleic acid; Detection; Digestion; DNA; DNA, Catalytic - chemistry; DNA, Catalytic - metabolism; enantiomers; Enzyme Stability; Enzymes; HeLa Cells; Humans; Ions; Ions - analysis; Ions - metabolism; Lead - analysis; Lead - metabolism; medicine; Metal ions; Metals; Nucleic acids; protein binding; Recognition; substrate specificity; Tumor Cells, Cultured

DNAzymes, an important type of metal ion-dependent functional nucleic acid, are widely applied in bioanalysis and biomedicine. However, the use of DNAzymes in practical applications has been impeded by the intrinsic drawbacks of natural nucleic acids, such as interferences from nuclease digestion an...