Identification of an anti-CRISPR protein that inhibits the CRISPR-Cas type I-B system in Clostridioides difficile

• Published in: mSphere Vol. 8; no. 6; p. e0040123
• Main Authors: Muzyukina, Polina, Shkaruta, Anton, Guzman, Noemi M., Andreani, Jessica, Borges, Adair L., Bondy-Denomy, Joseph, Maikova, Anna, Semenova, Ekaterina, Severinov, Konstantin, Soutourina, Olga
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 20.12.2023
• Subjects: Acr protein; Adaptation; Adaptive immunity; Amino acids; anti-CRISPR; Antibiotics; Arrays; Bacteriology; Bacteriophages - genetics; cas operons; Cloning; Clostridioides; Clostridioides difficile; Clostridioides difficile - genetics; CRISPR; CRISPR-Cas Systems; DNA mimicry; Efficiency; enteropathogen; Genes; Genomes; Inflammation; Nosocomial infection; Operons; Phages; Proteins; Research Article; type I-B CRISPR-Cas interference; anti-CRISPR; DNA mimicry; type I-B CRISPR-Cas interference; cas operons; Clostridioides difficile; enteropathogen; phage

CRISPR-Cas systems provide prokaryotic hosts with adaptive immunity against mobile genetic elements. Many bacteriophages encode anti-CRISPR (Acr) proteins that inhibit host defense. The identification of Acr proteins is challenging due to their small size and high sequence diversity, and only a limi...