Host Serine Proteases TMPRSS2 and TMPRSS11D Mediate Proteolytic Activation and Trypsin-Independent Infection in Group A Rotaviruses

• Published in: Journal of virology Vol. 95; no. 11
• Main Authors: Sasaki, Michihito, Itakura, Yukari, Kishimoto, Mai, Tabata, Koshiro, Uemura, Kentaro, Ito, Naoto, Sugiyama, Makoto, Wastika, Christida E., Orba, Yasuko, Sawa, Hirofumi
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 10.05.2021
• Subjects: Virus-Cell Interactions; viral entry; proteolytic activation; type II transmembrane serine proteases; group A rotavirus; VP4

Proteolytic cleavage of the viral VP4 protein is essential for virion maturation and infectivity in group A rotaviruses (RVAs). In cell culture, RVAs are propagated in culture medium supplemented with the exogenous protease trypsin, which cleaves VP4 and induces the maturation of progeny RVA virions...