Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy

Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determin...

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Published inAnnual review of biochemistry Vol. 91; no. 1; pp. 1 - 32
Main Authors Chua, Eugene Y.D, Mendez, Joshua H, Rapp, Micah, Ilca, Serban L, Tan, Yong Zi, Maruthi, Kashyap, Kuang, Huihui, Zimanyi, Christina M, Cheng, Anchi, Eng, Edward T, Noble, Alex J, Potter, Clinton S, Carragher, Bridget
Format Journal Article
LanguageEnglish
Published United States Annual Reviews 21.06.2022
Annual Reviews, Inc
Subjects
Antibodies
automation
COVID-19
cryo-EM
Cryoelectron Microscopy - methods
democratization
Electron microscopy
Humans
in situ tomography
machine learning
Pandemics
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Single Molecule Imaging
Transmission electron microscopy
World problems
SARS-CoV-2
democratization
automation
machine learning
cryo-EM
in situ tomography
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Summary:Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.
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These authors contributed equally to this article
ISSN:0066-4154
1545-4509
DOI:10.1146/annurev-biochem-032620-110705