Application of the protein semisynthesis strategy to the generation of modified chromatin

• Published in: Annual review of biochemistry Vol. 84; p. 265
• Main Authors: Holt, Matthew, Muir, Tom
• Format: Journal Article
• Language: English
• Published: United States 01.01.2015
• Subjects: Chromatin - metabolism; Histones - metabolism; Peptides - metabolism; Protein Engineering - methods; Protein Processing, Post-Translational; expressed protein ligation; histone; posttranslational modification; epigenetics

Histone proteins are subject to a host of posttranslational modifications (PTMs) that modulate chromatin structure and function. Such control is achieved by the direct alteration of the intrinsic physical properties of the chromatin fiber or by regulating the recruitment and activity of a host of tr...