Single Seed Microbiota: Assembly and Transmission from Parent Plant to Seedling

• Published in: mBio Vol. 13; no. 6; p. e0164822
• Main Authors: Chesneau, Guillaume, Laroche, Béatrice, Préveaux, Anne, Marais, Coralie, Briand, Martial, Marolleau, Brice, Simonin, Marie, Barret, Matthieu
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 20.12.2022
• Subjects: community assembly; Germination; Life Sciences; Microbial Ecology; Microbiota; neutral models; Research Article; seed; seedling transmission; Seedlings; Seeds - microbiology; selection; microbiota; neutral models; seedling transmission; seed; selection; community assembly; seed microbiota community assembly seedling transmission selection neutral models
• DOI: 10.1128/mbio.01648-22
• ISSN: 2150-7511; 2161-2129

Seeds are key components of plant fitness and are central to the sustainability of the agri-food system. Both the seed quality for food consumption and the seed vigor in agricultural settings can be influenced by the seed microbiota. The seed acts as the primary inoculum source for the plant microbiota. Understanding the processes involved in its assembly and dynamics during germination and seedling emergence has the potential to allow for the improvement of crop establishment. Changes in the bacterial community structure were tracked in 1,000 individual seeds that were collected throughout seed developments of beans and radishes. Seeds were associated with a dominant bacterial taxon that represented more than 75% of all reads. The identity of this taxon was highly variable between the plants and within the seeds of the same plant. We identified selection as the main ecological process governing the succession of dominant taxa during seed filling and maturation. In a second step, we evaluated the seedling transmission of seed-borne taxa in 160 individual plants. While the initial bacterial abundance on seeds was not a good predictor of seedling transmission, the identities of the seed-borne taxa modified the phenotypes of seedlings. Overall, this work revealed that individual seeds are colonized by a few bacterial taxa of highly variable identity, which appears to be important for the early stages of plant development. IMPORTANCE Seeds are key components of plant fitness and are central to the sustainability of the agri-food system. Both the seed quality for food consumption and the seed vigor in agricultural settings can be influenced by the seed microbiota. Understanding the ecological processes involved in seed microbiota assembly will inform future practices for promoting the presence of important seed microorganisms for plant health and productivity. Our results highlighted that seeds were associated with one dominant bacterial taxon of variable taxonomic identity. This variety of dominant taxa was due to (i) spatial heterogeneity between and within plants and (ii) primary succession during seed development. According to neutral models, selection was the main driver of microbial community assembly for both plant species.