Highly Reproducible Absolute Quantification of Mycobacterium tuberculosis Complex by Digital PCR

• Published in: Analytical chemistry (Washington) Vol. 87; no. 7; pp. 3706 - 3713
• Main Authors: Devonshire, Alison S, Honeyborne, Isobella, Gutteridge, Alice, Whale, Alexandra S, Nixon, Gavin, Wilson, Philip, Jones, Gerwyn, McHugh, Timothy D, Foy, Carole A, Huggett, Jim F
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.04.2015
• Subjects: Animals; Bacteria; Calibration; Chip formation; Deoxyribonucleic acid; Digital; DNA; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Droplets; Error analysis; Extraction; Format; genome; Genomes; Humans; Mixes; Molecular weight; Mycobacterium bovis - genetics; Mycobacterium Infections - microbiology; Mycobacterium tuberculosis; Mycobacterium tuberculosis - genetics; Nucleic acids; pathogens; Plasmids - genetics; Polymerase chain reaction; Polymerase Chain Reaction - methods; Reproducibility of Results; Tuberculosis; variance

Digital PCR (dPCR) offers absolute quantification through the limiting dilution of template nucleic acid molecules and has the potential to offer high reproducibility. However, the robustness of dPCR has yet to be evaluated using complex genomes to compare different dPCR methods and platforms. We us...