Analysis of Chemical Equilibrium of Silicon-Substituted Fluorescein and Its Application to Develop a Scaffold for Red Fluorescent Probes

• Published in: Analytical chemistry (Washington) Vol. 87; no. 17; pp. 9061 - 9069
• Main Authors: Hirabayashi, Kazuhisa, Hanaoka, Kenjiro, Takayanagi, Toshio, Toki, Yuko, Egawa, Takahiro, Kamiya, Mako, Komatsu, Toru, Ueno, Tasuku, Terai, Takuya, Yoshida, Kengo, Uchiyama, Masanobu, Nagano, Tetsuo, Urano, Yasuteru
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.09.2015
• Subjects: Absorption; Analogue; Analytical chemistry; beta-galactosidase; chemical analysis; chemical equilibrium; Chemical synthesis; Derivatives; Fluorescein; Fluorescein - chemical synthesis; Fluorescein - chemistry; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Hydrogen-Ion Concentration; Lactones; Molecular Structure; Organic chemicals; Photochemical Processes; Probes; Scaffolds; Silicon; Silicon - chemistry; Spectra

Fluorescein is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes. Here, we report synthesis and characterization of a silicon-substituted fluorescein, i.e., 2-COOH TokyoMagenta (2-COOH TM), which is a fluorescein analogue in whi...