Cyclometalated Iridium Complex-Based Label-Free Photoelectrochemical Biosensor for DNA Detection by Hybridization Chain Reaction Amplification

• Published in: Analytical chemistry (Washington) Vol. 87; no. 8; pp. 4283 - 4291
• Main Authors: Li, Chunxiang, Wang, Hongyang, Shen, Jing, Tang, Bo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.04.2015
• Subjects: Absorption spectroscopy; Amplification; Analytical chemistry; Biosensing Techniques; Biosensors; Chain reactions; Deoxyribonucleic acid; detection limit; Dissolved oxygen; DNA; DNA - analysis; Electrochemical Techniques; Electrodes; Fluorescence; fluorescence emission spectroscopy; Hybridization; Iridium; Iridium - chemistry; Molecular Structure; Nucleic Acid Hybridization; Organometallic Compounds - chemical synthesis; Organometallic Compounds - chemistry; Phenazines - chemical synthesis; Phenazines - chemistry; Photochemical Processes; Photoelectric effect; Sensors; Symbols; ultraviolet-visible spectroscopy

Photoactive material is the most crucial factor which intimately determines analytical performances of the photoelectrochemical sensor. On the basis of the high affinity of dipyrido [3,2-a:2′,3′-c] phenazine (dppz) with DNA helix, a novel photoactive intercalator, [(ppy)2Ir­(dppz)]+PF6 –(ppy = 2-phe...