Colorimetric Detection of Small Molecules in Complex Matrixes via Target-Mediated Growth of Aptamer-Functionalized Gold Nanoparticles

• Published in: Analytical chemistry (Washington) Vol. 87; no. 15; pp. 7644 - 7652
• Main Authors: Soh, Jun Hui, Lin, Yiyang, Rana, Subinoy, Ying, Jackie Y, Stevens, Molly M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.08.2015
• Subjects: Analytical chemistry; Aptamers, Nucleotide - chemistry; Assaying; Biosensing Techniques; Cocaine; Cocaine - chemistry; Cocaine - urine; Colorimetry; Colorimetry - methods; Desorption; detection limit; Detection limits; diagnostic techniques; drugs; estradiol; Estradiol - chemistry; Estradiol - urine; Food safety; Foods; Gold; Gold - chemistry; Humans; Limit of Detection; Metal Nanoparticles - chemistry; Molecules; nanogold; Nanoparticles; Nanostructure; Ochratoxin A; Ochratoxins - analysis; oligonucleotides; rapid methods; red wines; saliva; Saliva - chemistry; screening; Strands; Target detection; urine; Wine - analysis

A versatile and sensitive colorimetric assay that allows the rapid detection of small-molecule targets using the naked eye is demonstrated. The working principle of the assay integrates aptamer–target recognition and the aptamer-controlled growth of gold nanoparticles (Au NPs). Aptamer–target intera...