The Importance of the Leader Sequence for Directing Lanthionine Formation in Lacticin 481

• Published in: Biochemistry (Easton) Vol. 47; no. 28; pp. 7342 - 7351
• Main Authors: Patton, Gregory C, Paul, Moushumi, Cooper, Lisa E, Chatterjee, Champak, van der Donk, Wilfred A
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.07.2008
• Subjects: 5' Untranslated Regions - genetics; Alanine - analogs & derivatives; Alanine - biosynthesis; Alanine - metabolism; Amino Acid Sequence; Aminobutyrates - metabolism; Bacteriocins - biosynthesis; Bacteriocins - chemistry; Bacteriocins - genetics; Bacteriocins - metabolism; Conserved Sequence; DNA Primers; Enzymes - metabolism; Gene Expression; Molecular Sequence Data; Mutagenesis, Site-Directed; Plasmids; Sequence Alignment; Sulfides

Lantibiotics are post-translationally modified peptide antimicrobial agents that are synthesized with an N-terminal leader sequence and a C-terminal propeptide. Their maturation involves enzymatic dehydration of Ser and Thr residues in the precursor peptide to generate unsaturated amino acids, which...