A Rheological Study of the Association and Dynamics of MUC5AC Gels
The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC muc...
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Published in | Biomacromolecules Vol. 18; no. 11; pp. 3654 - 3664 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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United States
American Chemical Society
13.11.2017
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Abstract | The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micrometer-sized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully constituted mucus. |
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AbstractList | The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micrometer-sized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully constituted mucus. The details of how the mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micronsized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully-constituted mucus. |
Author | Rubinstein, Michael Wagner, Caroline E Turner, Bradley S McKinley, Gareth H Ribbeck, Katharina |
AuthorAffiliation | Department of Chemistry Massachusetts Institute of Technology Department of Mechanical Engineering University of North Carolina Department of Biological Engineering |
AuthorAffiliation_xml | – name: University of North Carolina – name: – name: Department of Mechanical Engineering – name: Department of Chemistry – name: Massachusetts Institute of Technology – name: Department of Biological Engineering – name: 1 Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 – name: 3 Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290 – name: 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 |
Author_xml | – sequence: 1 givenname: Caroline E orcidid: 0000-0001-5193-2797 surname: Wagner fullname: Wagner, Caroline E – sequence: 2 givenname: Bradley S surname: Turner fullname: Turner, Bradley S – sequence: 3 givenname: Michael surname: Rubinstein fullname: Rubinstein, Michael organization: University of North Carolina – sequence: 4 givenname: Gareth H surname: McKinley fullname: McKinley, Gareth H – sequence: 5 givenname: Katharina orcidid: 0000-0001-8260-338X surname: Ribbeck fullname: Ribbeck, Katharina email: ribbeck@mit.edu |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28903557$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1002/jmr.657 10.1021/bm0609691 10.1103/PhysRevE.88.022125 10.1007/978-3-642-34070-3 10.1073/pnas.0903438106 10.1016/j.jbiomech.2016.04.009 10.1002/polb.20207 10.1016/S0006-3495(99)77288-7 10.1016/j.bpj.2010.01.012 10.1146/annurev-fluid-121108-145608 10.1103/PhysRevLett.92.178101 10.1021/acs.biomac.6b00164 10.1103/PhysRevLett.74.1250 10.1002/mabi.201000137 10.1002/polb.1995.090330602 10.1371/journal.pone.0087681 10.1016/S0968-0004(01)02052-7 10.1098/rspa.2012.0284 10.1073/pnas.0903554106 10.1016/j.colsurfa.2012.03.001 10.3389/fimmu.2013.00310 10.1371/journal.pone.0069528 10.1039/C4SM02506G 10.1016/j.biomaterials.2013.01.064 10.1021/ma60033a018 10.1002/bip.22372 10.1126/science.1223012 10.1016/j.archoralbio.2007.06.009 10.1016/S0021-9258(20)71335-9 10.1016/j.cocis.2005.11.001 10.1016/S0370-1573(00)00070-3 10.1103/PhysRevLett.99.078301 10.1093/emboj/21.11.2664 10.1016/j.colsurfa.2006.03.024 10.1103/PhysRevB.12.2455 10.1088/1742-5468/2010/01/P01011 10.1021/ma0013049 10.1016/j.freeradbiomed.2004.07.027 10.1122/1.3532979 10.1039/fd9950100307 10.1016/j.exer.2007.01.018 10.1021/j100349a028 10.1007/s002890050021 10.1039/C4CP03465A 10.1073/pnas.1016325108 10.1021/bm401356q 10.1128/mBio.01911-14 10.1073/pnas.1311999110 |
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References | ref9/cit9 ref45/cit45 ref3/cit3 ref27/cit27 Smith B. F. (ref23/cit23) 1984; 259 ref16/cit16 ref52/cit52 ref8/cit8 ref31/cit31 ref2/cit2 ref34/cit34 ref37/cit37 ref20/cit20 ref48/cit48 ref17/cit17 ref10/cit10 ref35/cit35 ref19/cit19 ref21/cit21 ref42/cit42 ref46/cit46 ref49/cit49 ref13/cit13 ref24/cit24 ref50/cit50 ref6/cit6 ref36/cit36 ref18/cit18 Bhaskar K. R. (ref43/cit43) 1991; 261 Grodzinsky A. J. (ref38/cit38) 2008 ref25/cit25 ref29/cit29 ref32/cit32 ref39/cit39 ref14/cit14 ref5/cit5 ref51/cit51 ref28/cit28 ref40/cit40 ref26/cit26 ref12/cit12 ref15/cit15 ref41/cit41 ref22/cit22 ref33/cit33 Rubio R. G. (ref11/cit11) 2013 ref4/cit4 ref30/cit30 ref47/cit47 ref1/cit1 ref44/cit44 ref7/cit7 |
References_xml | – ident: ref42/cit42 doi: 10.1002/jmr.657 – ident: ref7/cit7 doi: 10.1021/bm0609691 – ident: ref35/cit35 doi: 10.1103/PhysRevE.88.022125 – volume-title: Without Bounds: A Scientific Canvas of Nonlinearity and Complex Dynamics year: 2013 ident: ref11/cit11 doi: 10.1007/978-3-642-34070-3 contributor: fullname: Rubio R. G. – ident: ref14/cit14 doi: 10.1073/pnas.0903438106 – ident: ref25/cit25 – ident: ref6/cit6 doi: 10.1016/j.jbiomech.2016.04.009 – ident: ref44/cit44 doi: 10.1002/polb.20207 – volume: 261 start-page: G827 year: 1991 ident: ref43/cit43 publication-title: Am. J. Physiol.: Gastrointest. Liver Physiol. contributor: fullname: Bhaskar K. R. – ident: ref40/cit40 doi: 10.1016/S0006-3495(99)77288-7 – ident: ref20/cit20 doi: 10.1016/j.bpj.2010.01.012 – ident: ref16/cit16 doi: 10.1146/annurev-fluid-121108-145608 – ident: ref32/cit32 doi: 10.1103/PhysRevLett.92.178101 – ident: ref49/cit49 doi: 10.1021/acs.biomac.6b00164 – ident: ref17/cit17 doi: 10.1103/PhysRevLett.74.1250 – volume-title: Fields Forces and Flows in Biological Systems year: 2008 ident: ref38/cit38 contributor: fullname: Grodzinsky A. J. – ident: ref24/cit24 doi: 10.1002/mabi.201000137 – ident: ref45/cit45 doi: 10.1002/polb.1995.090330602 – ident: ref18/cit18 doi: 10.1371/journal.pone.0087681 – ident: ref3/cit3 doi: 10.1016/S0968-0004(01)02052-7 – ident: ref15/cit15 doi: 10.1098/rspa.2012.0284 – ident: ref34/cit34 doi: 10.1073/pnas.0903554106 – ident: ref47/cit47 doi: 10.1016/j.colsurfa.2012.03.001 – ident: ref10/cit10 doi: 10.3389/fimmu.2013.00310 – ident: ref12/cit12 doi: 10.1371/journal.pone.0069528 – ident: ref29/cit29 doi: 10.1039/C4SM02506G – ident: ref48/cit48 doi: 10.1016/j.biomaterials.2013.01.064 – ident: ref41/cit41 doi: 10.1021/ma60033a018 – ident: ref19/cit19 doi: 10.1002/bip.22372 – ident: ref26/cit26 – ident: ref5/cit5 doi: 10.1126/science.1223012 – ident: ref1/cit1 doi: 10.1016/j.archoralbio.2007.06.009 – volume: 259 start-page: 12170 year: 1984 ident: ref23/cit23 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(20)71335-9 contributor: fullname: Smith B. F. – ident: ref2/cit2 doi: 10.1016/j.cocis.2005.11.001 – ident: ref28/cit28 doi: 10.1016/S0370-1573(00)00070-3 – ident: ref37/cit37 doi: 10.1103/PhysRevLett.99.078301 – ident: ref22/cit22 doi: 10.1093/emboj/21.11.2664 – ident: ref46/cit46 doi: 10.1016/j.colsurfa.2006.03.024 – ident: ref30/cit30 doi: 10.1103/PhysRevB.12.2455 – ident: ref31/cit31 doi: 10.1088/1742-5468/2010/01/P01011 – ident: ref39/cit39 doi: 10.1021/ma0013049 – ident: ref9/cit9 doi: 10.1016/j.freeradbiomed.2004.07.027 – ident: ref36/cit36 doi: 10.1122/1.3532979 – ident: ref50/cit50 doi: 10.1039/fd9950100307 – ident: ref4/cit4 doi: 10.1016/j.exer.2007.01.018 – ident: ref52/cit52 doi: 10.1021/j100349a028 – ident: ref51/cit51 doi: 10.1007/s002890050021 – ident: ref27/cit27 doi: 10.1039/C4CP03465A – ident: ref33/cit33 doi: 10.1073/pnas.1016325108 – ident: ref13/cit13 doi: 10.1021/bm401356q – ident: ref21/cit21 doi: 10.1128/mBio.01911-14 – ident: ref8/cit8 doi: 10.1073/pnas.1311999110 |
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Snippet | The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a... The details of how the mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a... |
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SubjectTerms | Animals Hydrogels - chemistry Hydrogen-Ion Concentration Mucin 5AC - chemistry Mucin 5AC - genetics Particle Size Polymers - chemistry Rheology Salts - chemistry Surface-Active Agents - chemistry Swine Viscosity |
Title | A Rheological Study of the Association and Dynamics of MUC5AC Gels |
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