A Rheological Study of the Association and Dynamics of MUC5AC Gels

The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC muc...

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Published inBiomacromolecules Vol. 18; no. 11; pp. 3654 - 3664
Main Authors Wagner, Caroline E, Turner, Bradley S, Rubinstein, Michael, McKinley, Gareth H, Ribbeck, Katharina
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 13.11.2017
Subjects
Animals
Hydrogels - chemistry
Hydrogen-Ion Concentration
Mucin 5AC - chemistry
Mucin 5AC - genetics
Particle Size
Polymers - chemistry
Rheology
Salts - chemistry
Surface-Active Agents - chemistry
Swine
Viscosity
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Abstract The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micrometer-sized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully constituted mucus.
AbstractList The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micrometer-sized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully constituted mucus.
The details of how the mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micronsized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully-constituted mucus.
Author Rubinstein, Michael
Wagner, Caroline E
Turner, Bradley S
McKinley, Gareth H
Ribbeck, Katharina
AuthorAffiliation Department of Chemistry
Massachusetts Institute of Technology
Department of Mechanical Engineering
University of North Carolina
Department of Biological Engineering
AuthorAffiliation_xml – name: University of North Carolina
– name:
– name: Department of Mechanical Engineering
– name: Department of Chemistry
– name: Massachusetts Institute of Technology
– name: Department of Biological Engineering
– name: 1 Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
– name: 3 Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290
– name: 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
Author_xml – sequence: 1
  givenname: Caroline E
  orcidid: 0000-0001-5193-2797
  surname: Wagner
  fullname: Wagner, Caroline E
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  surname: Turner
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  fullname: Rubinstein, Michael
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/28903557$$D View this record in MEDLINE/PubMed
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Snippet The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a...
The details of how the mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a...
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StartPage 3654
SubjectTerms Animals
Hydrogels - chemistry
Hydrogen-Ion Concentration
Mucin 5AC - chemistry
Mucin 5AC - genetics
Particle Size
Polymers - chemistry
Rheology
Salts - chemistry
Surface-Active Agents - chemistry
Swine
Viscosity
Title A Rheological Study of the Association and Dynamics of MUC5AC Gels
URI http://dx.doi.org/10.1021/acs.biomac.7b00809
https://www.ncbi.nlm.nih.gov/pubmed/28903557
https://search.proquest.com/docview/1938850961
https://pubmed.ncbi.nlm.nih.gov/PMC5776034
Volume 18
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