Label-Free LC–MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis

• Published in: Journal of proteome research Vol. 14; no. 11; pp. 4486 - 4501
• Main Authors: Collins, Mahlon A, An, Jiyan, Hood, Brian L, Conrads, Thomas P, Bowser, Robert P
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.11.2015
• Subjects: Adaptor Proteins, Signal Transducing - cerebrospinal fluid; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - isolation & purification; Adult; Alzheimer Disease - cerebrospinal fluid; Alzheimer Disease - diagnosis; Alzheimer Disease - genetics; Alzheimer Disease - pathology; Amyloid beta-Protein Precursor - cerebrospinal fluid; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - isolation & purification; amyotrophic lateral sclerosis; Amyotrophic Lateral Sclerosis - cerebrospinal fluid; Amyotrophic Lateral Sclerosis - diagnosis; Amyotrophic Lateral Sclerosis - genetics; Amyotrophic Lateral Sclerosis - pathology; biomarkers; Biomarkers - cerebrospinal fluid; Calcium-Binding Proteins - cerebrospinal fluid; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - isolation & purification; Case-Control Studies; cell adhesion molecules; Cell Adhesion Molecules - cerebrospinal fluid; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - isolation & purification; cerebrospinal fluid; Cerebrospinal Fluid Proteins - cerebrospinal fluid; Cerebrospinal Fluid Proteins - genetics; Cerebrospinal Fluid Proteins - isolation & purification; Chromatography, Liquid - methods; Diagnosis, Differential; extracellular matrix; Extracellular Matrix - chemistry; Extracellular Matrix Proteins - cerebrospinal fluid; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - isolation & purification; Humans; Immunoglobulins - cerebrospinal fluid; Immunoglobulins - genetics; Immunoglobulins - isolation & purification; Inflammation; liquid chromatography; Middle Aged; Motor Neuron Disease - cerebrospinal fluid; Motor Neuron Disease - diagnosis; Motor Neuron Disease - genetics; Motor Neuron Disease - pathology; neurons; proteome; Proteome - genetics; Proteome - isolation & purification; Proteome - metabolism; proteomics; Proteomics - methods; Sensitivity and Specificity; Support Vector Machine; support vector machines; Synapses - genetics; Synapses - metabolism; Synaptic Transmission; tandem mass spectrometry; Tandem Mass Spectrometry - methods; proteomics; cerebrospinal fluid; liquid chromatography-tandem mass spectrometry; mass spectrometry; biomarker; amyotrophic lateral sclerosis; extracellular matrix; LC−MS/MS

Analysis of the cerebrospinal fluid (CSF) proteome has proven valuable to the study of neurodegenerative disorders. To identify new protein/pathway alterations and candidate biomarkers for amyotrophic lateral sclerosis (ALS), we performed comparative proteomic profiling of CSF from sporadic ALS (sAL...