Label-Free Detection of Peptide Nucleic Acid−DNA Hybridization Using Localized Surface Plasmon Resonance Based Optical Biosensor

• Published in: Analytical chemistry (Washington) Vol. 77; no. 21; pp. 6976 - 6984
• Main Authors: Endo, Tatsuro, Kerman, Kagan, Nagatani, Naoki, Takamura, Yuzuru, Tamiya, Eiichi
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.11.2005
• Subjects: Analytical chemistry; Applied sciences; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Chemistry; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA Probes - chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Global environmental pollution; Hybridization; Methods. Procedures. Technologies; Nucleic Acid Hybridization; Peptide Nucleic Acids - chemistry; Peptides; Pollution; Sensitivity and Specificity; Spectrometric and optical methods; Surface Plasmon Resonance - methods; Various methods and equipments; Gold; Peptides; Nanoparticle; Glass; Immobilization; Chemical sensor; Silica; Protein; Thickness; Absorption spectrometry; Chemical modification; Biosensor; DNA; Detection limit; Optical properties; Environment; Oligonucleotide; Surface plasmon resonance; DNA hybridization; Nucleic acid; Medical application; Monitoring; Silane

The development of label-free optical biosensors for DNA and other biomolecules has the potential to impact life sciences as well as screening in medical and environmental applications. In this report, we developed a localized surface plasmon resonance (LSPR) based label-free optical biosensor based...