Label-Free Detection of Peptide Nucleic Acid−DNA Hybridization Using Localized Surface Plasmon Resonance Based Optical Biosensor

• Published in: Analytical chemistry (Washington) Vol. 77; no. 21; pp. 6976 - 6984
• Main Authors: Endo, Tatsuro, Kerman, Kagan, Nagatani, Naoki, Takamura, Yuzuru, Tamiya, Eiichi
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.11.2005
• Subjects: Analytical chemistry; Applied sciences; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Chemistry; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA Probes - chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Global environmental pollution; Hybridization; Methods. Procedures. Technologies; Nucleic Acid Hybridization; Peptide Nucleic Acids - chemistry; Peptides; Pollution; Sensitivity and Specificity; Spectrometric and optical methods; Surface Plasmon Resonance - methods; Various methods and equipments; Gold; Peptides; Nanoparticle; Glass; Immobilization; Chemical sensor; Silica; Protein; Thickness; Absorption spectrometry; Chemical modification; Biosensor; DNA; Detection limit; Optical properties; Environment; Oligonucleotide; Surface plasmon resonance; DNA hybridization; Nucleic acid; Medical application; Monitoring; Silane
• DOI: 10.1021/ac0513459
• ISSN: 0003-2700

The development of label-free optical biosensors for DNA and other biomolecules has the potential to impact life sciences as well as screening in medical and environmental applications. In this report, we developed a localized surface plasmon resonance (LSPR) based label-free optical biosensor based on a gold-capped nanoparticle layer substrate immobilized with peptide nucleic acids (PNAs). PNA probe was designed to recognize the target DNA related to tumor necrosis factor. The nanoparticle layer was formed on a gold-deposited glass substrate by the surface modified silica nanoparticles using silane-coupling reagent. The optical properties of gold-capped nanoparticle layer substrate were characterized through monitoring the changes in the absorbance strength, as the thickness of the biomolecular layer increased with hybridization. The detection of PNA−DNA hybridization with target oligonucleotides and PCR-amplified real samples were performed with a limit of detection value of 0.677 pM target DNA. Selective discrimination against a single-base mismatch was also achieved. Our LSPR-based biosensor with the gold-capped nanoparticle layer substrate is applicable to the design of biosensors for monitoring of the interaction of other biomolecules, such as proteins, whole cells, or receptors with a massively parallel detection capability in a highly miniaturized package.