Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue

• Published in: Journal of proteome research Vol. 13; no. 3; pp. 1757 - 1765
• Main Authors: Ma, Jiao, Ward, Carl C, Jungreis, Irwin, Slavoff, Sarah A, Schwaid, Adam G, Neveu, John, Budnik, Bogdan A, Kellis, Manolis, Saghatelian, Alan
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.03.2014
• Subjects: Breast Neoplasms - chemistry; Breast Neoplasms - genetics; Cell Line; cell lines; Chromatography, Liquid; Codon, Initiator - chemistry; Codon, Initiator - genetics; Female; Genome, Human; Humans; K562 Cells; molecular biology; Open Reading Frames; Peptides - analysis; Peptides - chemistry; polypeptides; Protein Biosynthesis; proteome; Proteome - analysis; Proteome - chemistry; start codon; Tandem Mass Spectrometry; peptidomics; short open reading frames (sORFs); sORF-encoded peptides (SEPs)

The existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection of their sORF-encoded polypeptide (SEP) products. The discovery of novel SEPs increases the size of the genome and the proteome and provides insights into the molecular...