Optimization of Low-Biomass Sample Collection and Quantitative PCR-Based Titration Impact 16S rRNA Microbiome Resolution

• Published in: Microbiology spectrum Vol. 10; no. 6; p. e0225522
• Main Authors: Clokie, Benjamin Gregory James, Elsheshtawy, Ahmed, Albalat, Amaya, Nylund, Are, Beveridge, Allan, Payne, Chris J., MacKenzie, Simon
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 21.12.2022
• Subjects: 16S rRNA; Animals; Bacteria - genetics; Biomass; complex; DNA Contamination; DNA, Bacterial - genetics; Ecology; equicopy; Fishes - genetics; gill; low-biomass; Microbiota - genetics; Polymerase Chain Reaction - methods; Research Article; RNA, Ribosomal, 16S - genetics; titration; 16S rRNA; equicopy; gill; titration; low-biomass; complex

The interaction between the fish gill and surrounding bacteria-rich water provides an intriguing model for examining the interaction between the fish, free-floating bacteria, and the bacterial microbiome on the gill surface. Samples that are inherently low in bacteria, or that have components that i...