Optimization of Low-Biomass Sample Collection and Quantitative PCR-Based Titration Impact 16S rRNA Microbiome Resolution - Clokie, Benjamin Gregory James; Elsheshtawy, Ahmed; Albalat, Amaya; Nylund, Are; Beveridge, Allan; Payne, Chris J.; MacKenzie, Simon (Microbiology spectrum, 2022)
Keywords: 16S rRNA; Animals; Bacteria - genetics; Biomass; complex; DNA Contamination; DNA, Bacterial - genetics; Ecology; equicopy; Fishes - genetics; gill; low-biomass; Microbiota - genetics; Polymerase Chain Reaction - methods; Research Article; RNA, Ribosomal, 16S - genetics; titration; 16S rRNA; equicopy; gill; titration; low-biomass; complex