Digital Deconvolution Filter Derived from Linear Discriminant Analysis and Application for Multiphoton Fluorescence Microscopy

• Published in: Analytical chemistry (Washington) Vol. 86; no. 7; pp. 3508 - 3516
• Main Authors: Sullivan, Shane Z, Schmitt, Paul D, Muir, Ryan D, DeWalt, Emma L, Simpson, Garth J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.04.2014
• Subjects: Atoms & subatomic particles; Counting; Digital; Discriminant Analysis; Distortion; Fluorescence; Impulses; Microscopy; Microscopy, Fluorescence - methods; Photons; Simulation

A digital filter derived from linear discriminant analysis (LDA) is developed for recovering impulse responses in photon counting from a high speed photodetector (rise time of ∼1 ns) and applied to remove ringing distortions from impedance mismatch in multiphoton fluorescence microscopy. Training of...