Separation of Long Double-Stranded DNA by Nanoparticle-Filled Capillary Electrophoresis

• Published in: Analytical chemistry (Washington) Vol. 76; no. 1; pp. 192 - 196
• Main Authors: Huang, Ming-Feng, Kuo, Yi-Chun, Huang, Chih-Ching, Chang, Huan-Tsung
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.01.2004
• Subjects: Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Capillarity; Cellulose; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromosomes; Deoxyribonucleic acid; DNA; DNA - isolation & purification; Dna, deoxyribonucleoproteins; Electrophoresis; Electrophoresis, Capillary - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Nanoparticles; Nanotechnology - methods; Nucleic acids; Other chromatographic methods; Phage ^l; Polymers; Ethylene oxide polymer; Gold; Double stranded DNA; Chemical modification; Efficiency; Capillary electrophoresis; Nanoparticle; Reproducibility; Molecular mass

We present the first example of the analysis of long double-stranded (ds) DNA molecules by nanoparticle-filled capillary electrophoresis (NFCE). To avoid aggregation of the gold nanoparticles (GNPs) and to allow strong interactions with the DNA molecules, the gold nanoparticles were modified with po...