Engineering Vascularized Organoid-on-a-Chip Models
Recreating human organ-level function in vitro is a rapidly evolving field that integrates tissue engineering, stem cell biology, and microfluidic technology to produce 3D organoids. A critical component of all organs is the vasculature. Herein, we discuss general strategies to create vascularized o...
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| Published in | Annual review of biomedical engineering Vol. 23; pp. 141 - 167 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Annual Reviews
13.07.2021
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| Abstract | Recreating human organ-level function in vitro is a rapidly evolving field that integrates tissue engineering, stem cell biology, and microfluidic technology to produce 3D organoids. A critical component of all organs is the vasculature. Herein, we discuss general strategies to create vascularized organoids, including common source materials, and survey previous work using vascularized organoids to recreate specific organ functions and simulate tumor progression. Vascularization is not only an essential component of individual organ function but also responsible for coupling the fate of all organs and their functions. While some success in coupling two or more organs together on a single platform has been demonstrated, we argue that the future of vascularized organoid technology lies in creating organoid systems complete with tissue-specific microvasculature and in coupling multiple organs through a dynamic vascular network to create systems that can respond to changing physiological conditions. |
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| AbstractList | Recreating human organ-level function in vitro is a rapidly evolving field that integrates tissue engineering, stem cell biology, and microfluidic technology to produce 3D organoids. A critical component of all organs is the vasculature. Herein, we discuss general strategies to create vascularized organoids, including common source materials, and survey previous work using vascularized organoids to recreate specific organ functions and simulate tumor progression. Vascularization is not only an essential component of individual organ function but also responsible for coupling the fate of all organs and their functions. While some success in coupling two or more organs together on a single platform has been demonstrated, we argue that the future of vascularized organoid technology lies in creating organoid systems complete with tissue-specific microvasculature and in coupling multiple organs through a dynamic vascular network to create systems that can respond to changing physiological conditions. Recreating human organ-level function in vitro is a rapidly evolving field that integrates tissue engineering, stem cell biology, and microfluidic technology to produce 3D organoids. A critical component of all organs is the vasculature. Herein, we discuss general strategies to create vascularized organoids, including common source materials, and survey previous work using vascularized organoids to recreate specific organ functions and simulate tumor progression. Vascularization is not only an essential component of individual organ function but also responsible for coupling the fate of all organs and their functions. While some success in coupling two or more organs together on a single platform has been demonstrated, we argue that the future of vascularized organoid technology lies in creating organoid systems complete with tissue-specific microvasculature and in coupling multiple organs through a dynamic vascular network to create systems that can respond to changing physiological conditions. Expected final online publication date for the , Volume 23 is June 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates. |
| Author | Hughes, Christopher C.W Shirure, Venktesh S George, Steven C |
| AuthorAffiliation | Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA 1 Department of Biomedical Engineering, University of California, Davis, California 95616, USA; email 2 scgeorge@ucdavis.edu |
| AuthorAffiliation_xml | – name: scgeorge@ucdavis.edu – name: Department of Biomedical Engineering, University of California, Davis, California 95616, USA; email – name: 2 – name: 1 – name: Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA |
| Author_xml | – sequence: 1 givenname: Venktesh S surname: Shirure fullname: Shirure, Venktesh S – sequence: 2 givenname: Christopher C.W surname: Hughes fullname: Hughes, Christopher C.W – sequence: 3 givenname: Steven C surname: George fullname: George, Steven C |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33756087$$D View this record in MEDLINE/PubMed |
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| Title | Engineering Vascularized Organoid-on-a-Chip Models |
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