Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV‑2 Variants with ACE2

The SARS-CoV-2 pandemic spurred numerous research endeavors to comprehend the virus and mitigate its global severity. Understanding the binding interface between the virus and human receptors is pivotal to these efforts and paramount to curbing infection and transmission. Here we employ atomic force...

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Published inACS Nanoscience Au Vol. 4; no. 2; pp. 136 - 145
Main Authors Ray, Ankita, Minh Tran, Thu Thi, Santos Natividade, Rita dos, Moreira, Rodrigo A., Simpson, Joshua D., Mohammed, Danahe, Koehler, Melanie, L Petitjean, Simon J., Zhang, Qingrong, Bureau, Fabrice, Gillet, Laurent, Poma, Adolfo B., Alsteens, David
Format Journal Article Web Resource
LanguageEnglish
Published United States American Chemical Society 17.04.2024
American Chemical Society (ACS)
Subjects
ACE2
Atomic force microscopy
Biochemistry, Molecular Biology
biolayer interferometry
Biomechanics
Biophysics
Biotechnology
Chemical Sciences
Chemistry (miscellaneous)
Computer Science
convalescent patient sera
Human health sciences
Immunologie & maladie infectieuse
Immunology & infectious disease
Life Sciences
Materials Science (miscellaneous)
Mechanics
Modeling and Simulation
Physics
RBD
SARS-CoV-2
Sciences de la santé humaine
Steered molecular dynamics
ACE2
Atomic force microscopy
SARS-CoV-2
RBD
biolayer interferometry
Steered molecular dynamics
convalescent patient sera
SARS-CoV-2 ACE2 RBD Atomic force microscopy Steered molecular dynamics biolayer interferometry convalescent patient sera
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Abstract The SARS-CoV-2 pandemic spurred numerous research endeavors to comprehend the virus and mitigate its global severity. Understanding the binding interface between the virus and human receptors is pivotal to these efforts and paramount to curbing infection and transmission. Here we employ atomic force microscopy and steered molecular dynamics simulation to explore SARS-CoV-2 receptor binding domain (RBD) variants and angiotensin-converting enzyme 2 (ACE2), examining the impact of mutations at key residues upon binding affinity. Our results show that the Omicron and Delta variants possess strengthened binding affinity in comparison to the Mu variant. Further, using sera from individuals either vaccinated or with acquired immunity following Delta strain infection, we assess the impact of immunity upon variant RBD/ACE2 complex formation. Single-molecule force spectroscopy analysis suggests that vaccination before infection may provide stronger protection across variants. These results underscore the need to monitor antigenic changes in order to continue developing innovative and effective SARS-CoV-2 abrogation strategies.
AbstractList The SARS-CoV-2 pandemic spurred numerous research endeavors to comprehend the virus and mitigate its global severity. Understanding the binding interface between the virus and human receptors is pivotal to these efforts and paramount to curbing infection and transmission. Here we employ atomic force microscopy and steered molecular dynamics simulation to explore SARS-CoV-2 receptor binding domain (RBD) variants and angiotensin-converting enzyme 2 (ACE2), examining the impact of mutations at key residues upon binding affinity. Our results show that the Omicron and Delta variants possess strengthened binding affinity in comparison to the Mu variant. Further, using sera from individuals either vaccinated or with acquired immunity following Delta strain infection, we assess the impact of immunity upon variant RBD/ACE2 complex formation. Single-molecule force spectroscopy analysis suggests that vaccination before infection may provide stronger protection across variants. These results underscore the need to monitor antigenic changes in order to continue developing innovative and effective SARS-CoV-2 abrogation strategies.
Author Bureau, Fabrice
Ray, Ankita
Santos Natividade, Rita dos
Koehler, Melanie
Zhang, Qingrong
Poma, Adolfo B.
Gillet, Laurent
Moreira, Rodrigo A.
L Petitjean, Simon J.
Alsteens, David
Mohammed, Danahe
Simpson, Joshua D.
Minh Tran, Thu Thi
AuthorAffiliation Faculty of Materials Science and Technology
Immunology-Vaccinology Lab of the Faculty of Veterinary Medicine
Liège University
Basque Center for Applied Mathematics
WELBIO department
Vietnam National University
Louvain Institute of Biomolecular Science and Technology
WEL Research Institute
University of ScienceVNU HCM
Laboratory of Cellular and Molecular Immunology, GIGA Institute
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Issue 2
Keywords ACE2
Atomic force microscopy
SARS-CoV-2
RBD
biolayer interferometry
Steered molecular dynamics
convalescent patient sera
SARS-CoV-2 ACE2 RBD Atomic force microscopy Steered molecular dynamics biolayer interferometry convalescent patient sera
Language English
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Snippet The SARS-CoV-2 pandemic spurred numerous research endeavors to comprehend the virus and mitigate its global severity. Understanding the binding interface...
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SubjectTerms ACE2
Atomic force microscopy
Biochemistry, Molecular Biology
biolayer interferometry
Biomechanics
Biophysics
Biotechnology
Chemical Sciences
Chemistry (miscellaneous)
Computer Science
convalescent patient sera
Human health sciences
Immunologie & maladie infectieuse
Immunology & infectious disease
Life Sciences
Materials Science (miscellaneous)
Mechanics
Modeling and Simulation
Physics
RBD
SARS-CoV-2
Sciences de la santé humaine
Steered molecular dynamics
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Title Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV‑2 Variants with ACE2
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